不同基原大黄药材鉴别:以大黄对照提取物为参照  

Identification of Different Original Rhubarb Medicinal Materials,Using Rhubarb Reference Extract as a Reference

作　　者：黄国凯[1] 陈海燕 郭隆钢 刘潇潇[1] 吴垠 赵岳锐 窦浩 吴群悦 Huang Guokai;Chen Haiyan;Guo Longgang;Liu Xiaoxiao;Wu Yin;Zhao Yuerui;Dou Hao;Wu Qunyue(Guangdong Institute for Drug Control,Guangzhou 510663,China;GuangzhouChromap Biotechnology Co.,Ltd.,Guangzhou 510663,China)

机构地区：[1]广东省药品检验所,广东广州510663 [2]广州科曼生物科技有限公司,广东广州510663

出　　处：《亚太传统医药》2025年第3期33-39,共7页Asia-Pacific Traditional Medicine

基　　金：广东省药品监督管理局科技创新项目(2022TDZ01)。

摘　　要：目的:以大黄对照提取物为参照,采用薄层色谱法和高效液相色谱法对3种不同基原的大黄药材进行鉴别。方法:分别以环己烷-乙酸乙酯-甲酸(10∶6∶0.1)和三氯甲烷-甲醇-水(8∶3∶0.3)为展开剂对不同基原大黄药材中的蒽醌苷元类成分和蒽醌苷类成分进行分析;采用高效液相色谱法对不同基原的大黄进行高效液相指纹图谱分析,以Kromasil C_(18)(4.6 mm×250 mm,5μm)为色谱柱,以乙腈和0.1%磷酸为流动相进行梯度洗脱,柱温为30℃,进样量为10μL,在269 nm波长下检测。结果:3种不同基原的大黄药材在相同薄层色谱和高效液相色谱条件下,蒽醌苷元类成分薄层色谱、蒽醌苷类成分薄层色谱及高效液相指纹图谱均存在明显差异,可依此进行准确鉴别。结论:经标准化制备的大黄对照提取物在大黄药材的基原鉴定中具有良好的适用性。Objective:To identify three different origins of rhubarb by thin layer chromatography and high performance liquid chromatography,using the extractive reference substance of rhubarb as a reference.Methods:Used cyclohexane-ethyl acetate-formic acid(10∶6∶0.1)and trichloromethane-methanol-water(8∶3∶0.3)as developing agents to analyze the anthraquinone glycoside components and anthraquinone glycoside components in different origins of rhubarb;High performance liquid chromatography was used to analyze the fingerprint spectra of rhubarb with different origins,the chromatographic column was Kromasil C_(18)(4.6mm×250mm,5μm),the mobile phase is acetonitrile-0.1% phosphoric acid,gradient elution,column temperature of 30℃,injection amount of 10μL,and detection wavelength of 269 nm.Results:Under these chromatographic conditions,there were significant differences in the anthraquinone glycoside components and anthraquinone glycoside components among the three different origins of rhubarb.It can be clearly distinguished.Conclusion:The standardized prepared rhubarb control extract has good applicability in the identification of the origin of rhubarb medicinal materials.

关 键 词：掌叶大黄 唐古特大黄 药用大黄 对照提取物 薄层色谱 指纹图谱 

分 类 号：R282.5[医药卫生—中药学]