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作 者:张佳诺 陈月华 王文瑀 赵明月 张慧[1] 康廷国[1] 宋慧鹏 Zhang Jianuo;Chen Yuehua;Wang Wenyu;Zhao Mingyue;Zhang Hui;Kang Tingguo;Song Huipeng(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China)
出 处:《亚太传统医药》2025年第3期58-62,共5页Asia-Pacific Traditional Medicine
基 金:辽宁省教育厅基本科研项目(JYTQN2023455,JYTMS20231826);辽宁中医药大学自然科学类青年项目(2021LZY023);沈阳市中青年科技创新人才培育专项U35拔尖青年项目(RC230846)。
摘 要:目的:基于“性状学-生物效应-超微量扫描-指纹图谱”方法,对两面针及其五种混伪品单面针、竹叶椒、花椒、飞龙掌血、功劳根进行鉴别。方法:通过光学显微镜观察6种药材粉末,采用超微量紫外扫描获得其提取物的最大吸收波长,通过酶标仪检测药材对乙酰胆碱酯酶、α-葡萄糖苷酶的抑制活性及DPPH自由基清除,通过高效液相色谱获得6种药材的指纹图谱。结果:通过外表皮花纹及颜色鉴别出两面针与飞龙掌血、功劳根,木部粗糙或光滑区分出两面针与竹叶椒、花椒;通过是否有晶鞘纤维及中柱鞘纤维区分出功劳根;通过生物活性鉴定的IC 50数量级区分出单面针;通过超微量紫外扫描获得的最大吸收波长及指纹图谱分开花椒、竹叶椒、飞龙掌血及功劳根。结论:该研究不仅全面综合地鉴别了6种药材、确保了两面针药材的质量,也为两面针的临床合理应用提供保障,对日后两面针的鉴别及质量评价、生物效应评价提供参考。Objective:The identification of Zanthoxylum nitidum(Roxb.)DC.and its five adulterants Zanthoxylum dissitum Hemsl,Zanthoxylum armatum,Zanthoxylum bungeanum,Toddalia asiatica(L.)Lam.,and Mahonia bealei(Fort.)Carr will be conducted based on the“morphology-biological effect-ultra-trace scanning-fingerprint”method.Methods:The powders of 6 medicinal materials were observed under an optical microscope.The maximum absorption wavelength of the extracts was determined through ultra-micro UV scanning.Their inhibitory activities against acetylcholinesterase,α-glucosidase,and DPPH radical scavenging were detected using an enzyme-labeled instrument.Fingerprint was obtained through high-performance liquid chromatography.Results:Identifying exocuticle patterns and color distinguishes Zanthoxylum nitidum from Toddalia asiatica and Mahonia bealei.The rough or smooth xylem distinguishes Zanthoxylum nitidum from Zanthoxylum armatum and Zanthoxylum bungeanum.In microscopic identification,Mahonia bealei was distinguished based on the crystalline sheath fiber and pericycle fiber.The IC 50 magnitude was used to distinguish Zanthoxylum dissitum in biological activity identification.The maximum absorption wavelength obtained through ultra-micro UV scanning and fingerprint distinguishes between Zanthoxylum armatum,Zanthoxylum bungeanum,Toddalia asiatica,and Mahonia bealei.Conclusion:This study has comprehensively identified 6 medicinal materials,ensuring the quality of Zanthoxyli Radix,and provided a guarantee for the clinically rational application of Zanthoxylum nitidum.It laid a foundation for the future identification,quality evaluation,and biological effect evaluation of Zanthoxylum nitidum.
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