Interactive effects of bacteria-loaded biochar on the physiological responses of Brassica rapa var.chinensis in the Pb and Zn contaminated soil  

细菌胶团和生物炭联合修复对铅性复合污染土壤的小青菜生理作用效应

作　　者：LI Xue ZHU Xiao-li ZHU Feng LI Xing ZHANG Zi-ye XUE Sheng-guo 李雪;朱晓丽;朱锋;李星;张子夜;薛生国(School of City and Environment,Northwest University,Xi’an 710127,China;School of Metallurgy and Environment,Central South University,Changsha 410083,China;Hunan HIKEE Environmental Technology Co.,Ltd.,Changsha 410221,China;Xi’an Jinborui Ecological Tech.Co.,Ltd.,Xi’an 710065,China)

机构地区：[1]School of City and Environment,Northwest University,Xi’an 710127,China [2]School of Metallurgy and Environment,Central South University,Changsha 410083,China [3]Hunan HIKEE Environmental Technology Co.,Ltd.,Changsha 410221,China [4]Xi’an Jinborui Ecological Tech.Co.,Ltd.,Xi’an 710065,China

出　　处：《Journal of Central South University》2025年第1期149-159,共11页中南大学学报(英文版)

基　　金：Projects(2019NY-200,2020ZDLNY06-06,2020ZDLNY07-10)supported by the Key Research and Development Program of Shaanxi Province,China;Project(2019YFC1803604)supported by the National Key Research and Development Program of China。

摘　　要：Lead(Pb)and zinc(Zn)are widely recognized as common environmental contaminants,contributing to soil degradation and posing risks to environmental health.Combining functional carbon-based materials with microorganisms has been considered as an effective and environmentally friendly strategy for remediating Pb/Zn-contaminated soil.However,there is still a lack of understanding the connection between heavy metal immobilization and plant responses,which hampers practical applications.Here,a 90-day pot experiment was conducted to investigate the integrated effects of biochar(WS700)and microorganisms including inorganic phosphate-solubilizing bacteria(IPSB)and sulfate reducing bacteria(SRB)on Pb and Zn synchronous immobilization and the physiological responses of Brassica rapa var.chinensis(Brassica).Compared with CK,bacteria-loaded biochar treatment declined the exchangeable Pb and Zn fraction by 94.69%−98.37%and 94.55%−99.52%,while increasing the residual state Pb and Zn by 75.50%−208.58%and 96.71%−110.85%,respectively.Three amendments enhanced Brassica growth by improving total chlorophyll content and superoxide dismutase(SOD)and peroxidase(POD)activities.The bacteria-loaded biochar treatment effectively regulated stomatal conductance and reduced intercellular CO_(2) concentration.Moreover,compared with CK,three amendments reduced MDA content by 28.84%,28.30%and 41.60%,respectively,under the high concentration of Pb and Zn.The findings demonstrated the significant role of bacterial-biochar consortia in immobilizing Pb and Zn and mitigating Pb and Zn-induced stress in plants by regulating photosynthetic characteristics and antioxidant enzyme activities.铅(Pb)和锌(Zn)被广泛认为是常见的环境污染物,会导致土壤退化并对环境健康造成危害。功能性碳基材料与微生物相结合修复策略,被认为是修复铅和锌污染土壤有效且环保的策略。然而,碳基微生物修复剂作用下土壤铅锌钝化与植物生长效应之间的相互作用机理仍需厘清。本文采用90天盆栽试验,研究了生物炭(WS700)、解无机溶磷菌(IPSB)和硫酸盐还原菌(SRB)及固定化菌胶团对复合铅锌固定化的影响,并探究了其对小青菜(Brassica rapa var.chinensis,Brassica)的生理生化作用效果。与对照组相比,复合菌胶团负载生物炭分别降低了94.69%-98.37%和94.55%-99.52%的可交换态Pb和Zn,且同时提高了75.50%-208.58%和96.71%-110.85%的残渣态Pb和Zn。相同的处理和浓度梯度下,铅和锌的铁锰氧化物和有机组分呈现出相反的趋势。此外,三种修复剂通过提高总叶绿素含量、SOD和POD活性减轻对小青菜光合器官的损害、对Pb和Zn的吸收并促进其生长。复合细菌胶团负载生物炭刺激小青菜调节气孔导度并且降低自身胞间CO_(2)浓度,提高净光合作用速率来响应Pb和Zn的毒害作用。与对照组相比,在高浓度Pb和Zn污染下三种修复剂使MDA含量分别降低了28.84%、28.30%和41.60%,这表明复合细菌胶团负载生物炭可以通过调节光合作用特性和抗氧化酶活性,降低铅/锌对植物的毒害作用。

关 键 词：BIOCHAR immobilized bacteria consortia photosynthetic properties Pb and Zn immobilization antioxidant enzymes 

分 类 号：S15[农业科学—土壤学]