动态对比增强磁共振成像评估兔肢体高压电烧伤后早期骨骼肌微循环灌注的可行性研究  

Research on the feasibility of dynamic contrast-enhanced magnetic resonance imaging in assessing the microcirculatory perfusion of skeletal muscle in rabbit limbs in the early stage after high-voltage electric burns

作　　者：阮鹏 孙斯琴 葛映红 查云飞[2] Ruan Peng;Sun Siqin;Ge Yinghong;Zha Yunfei(Department of Radiology,Wuhan Third Hospital(Tongren Hospital of Wuhan University),Wuhan 430060,China;Department of Radiology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉市第三医院(武汉大学同仁医院)放射科,武汉430060 [2]武汉大学人民医院放射科,武汉430060

出　　处：《中华烧伤与创面修复杂志》2025年第2期163-170,共8页Chinese Journal of Burns And Wounds

基　　金：湖北省科技厅中央引导地方科技发展专项(2022BGE264);武汉市科技局知识创新专项(2022020801020551)。

摘　　要：目的 探讨动态对比增强磁共振成像(DCE-MRI)评估兔肢体高压电烧伤后早期骨骼肌微循环灌注的可行性.方法 该研究为实验研究.取32只6~8个月龄雄性大耳白兔,采用随机数字表法将兔分为右下肢致高压电烧伤的电烧伤组(20只)及右下肢致假伤的对照组(12只).伤后0.5、24.0、48.0、72.0 h,完成2组兔下肢轴位快速自旋回波T1加权成像、快速自旋回波T2加权成像及DCE-MRI检查,应用参考区域血流动力学模型获取损伤肢体骨骼肌血流灌注参数,包括容量转移常数K_(trans)值、速率常数K_(ep)值.取电烧伤组兔伤后0.5、24.0、48.0、72.0 h及对照组兔伤后0.5 h的骨骼肌组织,行免疫组织化学染色观察微血管情况并计算微血管密度(MVD).分析电烧伤组兔伤后0.5~72.0 h骨骼肌组织K_(trans)值、K_(ep)值与MVD的相关性.电烧伤组样本数为5,对照组样本数为3.结果 电烧伤组兔骨骼肌组织K_(trans)值和K_(ep)值均在伤后0.5-72.0 h呈先升高后降低的趋势,且在伤后24.0 h达到峰值.电烧伤组兔伤后0.5、24.0、48.0、72.0 h骨骼肌组织K_(trans)值(t值分别为-15.77、-14.91、-40.35、-40.25,P<0.05)及伤后 0.5、24.0、48.0 h 骨骼肌组织K_(ep)值(t 值分别为-5.39、-6.82、-6.83,P<0.05)均明显高于对照组.对照组兔伤后0.5 h及电烧伤组兔伤后0.5、24.0、48.0、72.0 h骨骼肌组织中 MVD分别为(24.7±3.5)、(21.8±2.2)、(40.8±9.1)、(16.4±2.4)、(9.8±0.8)根/mm^(2).电烧伤组兔伤后24.0 h骨骼肌组织中MVD明显高于对照组伤后0.5 h(t=2.89,P<0.05),伤后48.0、72.0 h骨骼肌组织中MVD均明显低于对照组伤后0.5 h(t值分别为4.01、9.52,P<0.05).电烧伤组兔伤后0.5~72.0 h骨骼肌组织中K_(trans)值、K_(ep)值均与MVD呈显著正相关(r值均为0.95,P<0.05).结论 DCE-MRI定量灌注参数K_(trans)值及K_(ep)值可有效反映兔肢体高压电烧伤后早期骨骼肌微循环灌注变化.ObjectiveTo explore the feasibility of dynamic contrast-enhanced magnetic resonance imaging(DCE-MRI)in assessing the microcirculatory perfusion of skeletal muscle in rabbit limbs in the early stage after high-voltage electric burns.MethodsThis study was an experimental study.Thirty-two male big ear white rabbits aged 6-8 months were assigned into electric burn group of 20 rabbits with high-voltage electric burns in the right lower limb and control group of 12 rabbits with sham injury in the right lower limb using the random number table method.At 0.5,24.0,48.0,and 72.0 h post injury,the lower limbs of rabbits in the two groups underwent axial fast spin echo T1-weighted imaging,fast spin echo T2-weighted imaging,and DCE-MRI examination.A reference region-based hemodynamic model was applied to obtain the blood perfusion parameters of skeletal muscle in the injured limbs,including the volume transfer constant K trans value and rate constant K ep value.The skeletal muscle tissue from the electric burn group of rabbits at 0.5,24.0,48.0,and 72.0 h post injury and the control group of rabbits at 0.5 h post injury was harvested for immunohistochemical staining to observe the microvascular changes and calculate the microvascular density(MVD).The correlation between the K trans and K ep values and the MVD of skeletal muscle tissue in electric burn group of rabbits at 0.5-72.0 h post injury was analyzed.The number of samples was 5 in the electric burn group,and the number of samples was 3 in the control burn group.ResultsFrom 0.5-72.0 h post injury,the K trans and K ep values in skeletal muscle tissue of electric burn group of rabbits exhibited the trends of increase first and decrease then,both of which reached peak values at 24.0 h post injury.The K trans values at 0.5,24.0,48.0,and 72.0 h post injury(with t values of-15.77,-14.91,-40.35,and-40.25,respectively,P<0.05)and the K ep values at 0.5,24.0,and 48.0 h post injury(with t values of-5.39,-6.82,and-6.83,respectively,P<0.05)in skeletal muscle tissue in electric burn gr

关 键 词：烧伤 电 磁共振成像 肌 骨骼 微血管 微循环 

分 类 号：R73[医药卫生—肿瘤]