环介导等温扩增技术及其在分子POCT产品中的应用  

Research Progress on Loop-mediated Isothermal Amplification(LAMP)Technology and Its Application to Molecular Point-of-care Testing(POCT)Products

作　　者：贺佳佳 孙睿 陈名利[2] 尹焕才[2] HE Jiajia;SUN Rui;CHEN Mingli;YIN Huancai(School of Biomedical Engineering(Suzhou),University of Science and Technology of China,Suzhou 215613,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215613,China)

机构地区：[1]中国科学技术大学生物医学工程学院(苏州),苏州215613 [2]中国科学院苏州生物医学工程技术研究所,苏州215613

出　　处：《中国生物工程杂志》2025年第1期38-48,共11页China Biotechnology

基　　金：国家重点研发计划(2022YFF0710102);江苏省社会发展项目(BE2022740);苏州市基础研究(SJC2022014)资助项目。

摘　　要：环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)反应速度较快、特异性良好、硬件需求低,常作为核酸扩增的核心方法用于核酸分子的即时检测(point-of-care testing,POCT)产品中。等温扩增分子POCT产品按检测流程通常分为样品前处理、核酸分子扩增和产物检测三个核心单元,每个单元环环相扣,决定产品性能及临床应用中的表现。在新冠疫情期间,LAMP及其衍生技术从实验室到临床得到快速发展,涌现了大量的分子POCT产品,从解决方案、成本和效益等方面加速了核酸检测技术的发展,推动了该技术在居家、社区、海关、野外等场景下的应用。综述等温扩增分子POCT产品三个核心单元的技术发展以及2019年以来用于新冠病毒及其他病原体检测的等温扩增分子POCT产品的原理、组成、产品性能和应用成效,探讨LAMP与微流控、免疫侧向流层析、成簇规律间隔短回文重复序列(clustered regularly interspaced short palindromic repeats,CRISPR)等技术的结合及未来产品开发趋势。Loop-mediated isothermal amplification(LAMP)is recognized for its rapid reaction time,high specificity,and low hardware requirements,and is often used as the core method for nucleic acid amplification in point-of-care testing(POCT)products for molecular nucleic acid detection.The molecular POCT products based on isothermal amplification consist of three core units according to the detection process:sample pretreatment,nucleic acid molecular amplification,and product detection.Each unit is interlinked,which determines the performance of the product and its performance in clinical applications.During the COVID-19 pandemic,LAMP and its derivative technologies moved rapidly from the laboratory to the clinic,leading to a surge in the development of numerous molecular POCT products.These advancements have accelerated the development of nucleic acid detection technology in terms of solutions,cost,and effectiveness,promoting the application of this technology in various scenarios such as home,community,customs,and field settings,ushering in a period of rapid growth.This article first provides an overview of the latest technological development of the three core units of isothermal amplification molecular POCT products.It also reviews the principles,composition,product performance,and application effectiveness of isothermal amplification molecular POCT products for the detection of SARS-CoV-2 and other pathogens since 2019.Additionally,it discusses the current state of technology integration and future product development trends of LAMP with microfluidics,immunochromatography lateral flow assays,and clustered regularly interspaced short palindromic repeats(CRISPR).

关 键 词：环介导等温扩增技术 即时检测 POCT产品 

分 类 号：Q819[生物学—生物工程]