高卢蜜环菌共生猪苓多孔菌菌核际土壤的蛋白组研究Ⅰ  

Sclerosphere soil proteome of Polyporus umbellatus during symbiosis with Armillaria gallicaⅠ

作　　者：李兵[1,2] 周凌峰 刘柳 郭顺星 LI Bing;ZHOU Lingfeng;LIU Liu;GUO Shunxing(Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,Beijing 100700,China)

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]道地药材品质保障与资源持续利用全国重点实验室,北京100700

出　　处：《菌物学报》2025年第2期44-54,共11页Mycosystema

基　　金：中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031,2022-I2M-2-001);中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302)。

摘　　要：为研究猪苓多孔菌与高卢蜜环菌互作过程中的菌核际土壤中蛋白表达及变化情况,本研究在陕西省留坝县进行了栽培试验,并对空白对照组(CK)、蜜环菌组(CKA)、猪苓多孔菌组(CKP)和实验组(T_(P+A))菌核际土壤中的蛋白进行了高分辨质谱鉴定与生物信息学分析。结果显示,猪苓多孔菌与高卢蜜环菌的互作,会改变彼此在菌核际中的蛋白表达谱。蛋白功能分析结果表明,未共生的高卢蜜环菌分泌蛋白可发挥催化和转移酶活性,参与磷脂酰肌醇信号系统、GABA信号等代谢途径;共生后,则特异性释放硫氧还蛋白样蛋白,发挥抗氧化和效应因子活性。未与高卢蜜环菌共生的猪苓多孔菌菌核并非完全的休眠结构,可以释放参与氨基酸、牛磺酸、磷酸盐等代谢的酶类物质,而囊泡运输中SNARE的相互作用可能在调控非共生的菌核与菌核际中物质的交换与运输中发挥着重要作用。与蜜环菌共生后,猪苓多孔菌菌核可能通过门控离子通道及钙离子信号转导等多种途径适应这种共生。本研究不仅加深了对猪苓多孔菌与蜜环菌互作的认知,也为二者共生机制的研究提供了参考。The exploration of expression and changes of sclerosphere soil proteome of Polyporus umbellatus during symbiosis with Armillaria gallica was conducted in Liuba County,Shaanxi Province.Four experimental groups were set up including blank control group(CK),non-symbiotic A.gallica group(CKA),non-symbiotic sclerotigenic P.umbellatus group(CKP)and experimental A.gallica and P.umbellatus simbiotic group(T_(P+A)).Proteomics analysis was carried out and high resolution mass spectrometry was applied to identify proteins from these groups.The results showed that the interaction between P.umbellatus and A.gallica could change the protein expression profile of each other in the sclerosphere.The informatics analysis indicated that the released protein of non-symbiotic A.gallica played catalytic and transferase activities,and participated in the phosphatidylinositol signaling system,GABA signal and other metabolic pathways;thioredoxin-like protein was specifically expressed by symbiotic A.gallica to exert antioxidant and effector activities.P.umbellatus sclerotium is not a complete resting structure,and can release enzymes involved in the metabolism of amino acids,taurine,phosphonate,etc.SNARE interactions in vesicular transport may play an important role in regulating the exchange and transport of substances between sclerotia and sclerosphere.After infection of A.gallica,sclerotia may adapt to symbiotic relationship through activated mechanosensitive ion channel and calcium signal transduction.This study provides valuable references for further research on mutualistic mechanisms of symbiosis between P.umbellatus and Armillaria sp.

关 键 词：猪苓多孔菌 高卢蜜环菌 菌核 菌核际 蛋白质组 

分 类 号：S154.3[农业科学—土壤学]