组蛋白赖氨酸甲基转移酶CfDot1在果生刺盘孢中的功能  

Function of histone lysine methyltransferase CfDot1 in Colletotrichum fructicola

作　　者：范晶晶 李冠辰 王子瑄 李河[1] FAN Jingjing;LI Guanchen;WANG Zixuan;LI He(Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation,Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests,Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]中南林业科技大学,南方人工林病虫害防控国家林草局重点实验室,森林有害生物防控湖南省重点实验室,经济林培育与保护教育部重点实验室,湖南长沙410004

出　　处：《菌物学报》2025年第2期55-67,共13页Mycosystema

基　　金：国家重点研发计划(2023YFD1401301)。

摘　　要：果生刺盘孢Colletotrichum fructicola是油茶炭疽病的优势致病菌。研究组蛋白赖氨酸甲基转移酶CfDot1在果生刺盘孢中的功能,为阐明其致病机制提供理论依据。基于反向遗传学理论,采用over-lap PCR构建基因敲除载体和回补质粒,进一步通过遗传转化、抗生素筛选和PCR扩增验证获得突变体菌株ΔCfdot1和回补菌株ΔCfdot1/CfDOT1。研究发现CfDot1定位于细胞核。生物学表型测定结果表明,与野生型和回补菌株相比,CfDOT1基因敲除突变体生长速率无明显变化,但其附着胞形成率显著降低,对细胞壁、H_(2)O_(2)以及内质网压力胁迫剂敏感,致病力显著减弱。组蛋白甲基转移酶CfDot1参与调控果生刺盘孢附着胞形成、外界胁迫应答及致病过程。Colletotrichum fructicola is the principal pathogen responsible for anthracnose in Camellia oleifera.Investigating histone lysine methyltransferase CfDot1 in C.fructicola lays the groundwork for understanding its role in pathogenicity.Based on reverse genetics principles,knockout and complement plasmids were constructed by using over-lap PCR method.The mutant strainΔCfdot1 and the complement strainΔCfdot1/CfDOT1 were generated through genetic transformation,antibiotics screening and PCR amplification validation.Subsequent analysis revealed the nuclear localization of CfDot1.Biological phenotype determination indicates that the growth rate of the CfDOT1 gene knockout mutant does not exhibit significant difference in comparison with the wild-type and complement strains.However,there was a notable decrease in appressorium formation rate,as well as hypersensitivity to cell wall,H_(2)O_(2),and endoplasmic reticulum stress agents,and therefore weakening pathogenicity.The histone methyltransferase CfDot1 plays a crucial role in regulation of spore production,appressorium formation,response to external stressors,and pathogenicity.

关 键 词：油茶 果生刺盘孢 组蛋白赖氨酸甲基转移酶CfDot1 致病力 

分 类 号：S763.7[农业科学—森林保护学]