灵芝子实体中粗多糖的制备及其抑制血管生成活性初步评价  

Preparation of crude polysaccharides from Ganoderma lingzhi and preliminary evaluation of its angiogenic inhibitory activities

作　　者：王庆福 黄清铧 王丽宁 胡彪 平兆华 孟飞 WANG Qingfu;HUANG Qinghua;WANG Lining;HU Biao;PING Zhaohua;MENG Fei(Guangdong Plant Fiber Comprehensive Utilization Engineering Technology Research and Development Center,Guangdong Engineering Laboratory of Biomass High-Value Utilization,Guangzhou Key Laboratory of Biomass Comprehensive Utilization,Research Development and Application Collaborative Innovation Center for Pharmaceutical and Food Homologous Efficacy Ingredients,Industry Innovation Base of Characteristic Agricultural Products Deep Processing,Institute of Biological and Medical Engineering,Guangdong Academy of Sciences,Guangzhou 510316,Guangdong,China)

机构地区：[1]广东省科学院生物与医学工程研究所,广东省植物纤维综合利用工程技术研究开发中心,广东省生物质高值化利用工程实验室,广州市植物纤维综合利用重点实验室,药食同源功效成分研发与应用协同创新中心,特色农产品深加工产业创新基地,广东广州510316

出　　处：《菌物学报》2025年第2期113-124,共12页Mycosystema

基　　金：广东省科学院青年人才专项(2023GDASQNRC-0306);广东省科学院发展专项资金项目(2024GDASZH-2024010102);广州市科技计划(2024E04J0947);云浮市科技计划(2022010202)。

摘　　要：本研究以甘蔗渣栽培的灵芝GL0102子实体为材料,采用微波-超声波协同提取粗多糖,经Sevag法去蛋白,透析后获得一灵芝多糖GL-P,并分析GL-P组分的理化特征、抗氧化活性及对斑马鱼腹腔静脉血管生成进行评价。研究表明,GL-P得率为(22.51±1.29)g/kg,其多糖含量为(65.41±2.08)%,蛋白质含量高达(31.46±0.34)%,分子量为(1.27±0.09)×10^(4)g/mol。单糖组成主要为木糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖,其中木糖和阿拉伯糖含量占一半以上。红外光谱分析表明,GL-P属β-型葡聚糖构型的多糖-蛋白质复合物。体外抗氧化活性评价显示,GL-P具有一定的抗氧化活性,2 mg/mL GL-P抗氧化效果与0.06–0.08 mg/mL VC相当。GL-P对斑马鱼鱼胚的安全浓度范围应小于4.0 mg/mL,GL-P对斑马鱼胚胎肠下静脉血管具有显著的抑制作用。本研究可为灵芝多糖开发为新型高效低毒的潜在抗肿瘤产品提供参考。Polysaccharide named as GL-P extracted from fruiting body of Ganoderma lingzhi GL0102 cultivated on bagasse was extracted by microwave-ultrasound and deproteinized by Sevag method and finally obtained through dialysis.The physicochemical characteristics and antioxidant activity of GL-P were analyzed,and its effect on angiogenesis of zebrafish peritoneal vein was evaluated.The results showed that the yield of GL-P was(22.51±1.29)g/kg.The polysaccharide content was(65.41±2.08)%,the protein content was(31.46±0.34)%,and the molecular weight was 1.27×10^(4) g/mol.The monosaccharide composition was mainly xylose,arabinose,mannose,glucose and galactose,of which xylose and arabinose accounted for more than a half.The infrared spectrum analysis showed that GL-P was a polysaccharide-protein complex with β-glucan configuration.In vitro antioxidant activity evaluation showed that GL-P had antioxidant activity,and the antioxidant effect of 2 mg/mL GL-P was equivalent to that of 0.06–0.08 mg/mL VC.The safe concentration range of GL-P for zebrafish embryos should be less than 4.0 mg/mL,and GL-P can significantly inhibit the subintestinal veins of zebrafish embryos.This study provides reference for the development of G.lingzhi polysaccharide as a new high-efficiency and low-toxicity potential anti-tumor product.

关 键 词：灵芝 多糖 单糖组成 抗氧化 血管生成 

分 类 号：R284.1[医药卫生—中药学]