基于HPLC技术对哈蟆油产地的研究  

Study on Traceability System of Ranae Oviductus HPLC

作　　者：聂丹丹 刘佳琦 隋冬梅 马丹 杨爱馥 翟亚楠 于淼淼 姜佳颖 NIE Dan-Dan;LIU Jia-Qi;SUI Dong-Mei;MA Dan;YANG Ai-Fu;ZHAI Ya-Nan;YU Miao-Miao;JIANG Jia-Ying(Changchun Customs Technology Center,Changchun 130062;Science and Technology Research Center of China Customs,Beijing 100026;Dalian Customs Technology Center,Dalian 116001)

机构地区：[1]长春海关技术中心,长春130062 [2]中国海关科学技术研究中心,北京100026 [3]大连海关技术中心,大连116001

出　　处：《中国口岸科学技术》2024年第S2期107-112,共6页China Port Science and Technology

基　　金：吉林省中医药科技项目(2024149,2023181);1+X合作单位资助

摘　　要：本研究采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)对不同产地的哈蟆油样品及其伪品进行1-甲基海因、胞嘧啶和5-甲基胞嘧啶的测定。鉴于基因组DNA中5m C的含量能够体现DNA甲基化水平,因此可通过计算积分面积5mC/(5mC+C)的百分数来测定。结果显示,应用HPLC测定1-甲基海因含量和样品基因组胞嘧啶含量,可用于区分哈蟆油和其他伪品,不同产地间无显著性。The determination of 1-methylhydantoin,cytosine and 5-methylcytosine of Ranae Oviductus and its forgeries from different origin was carried out by HPLC(High Performance Liquid Chromatography).The content of 5mC in genomic DNA can reflect the level of DNA methylation,which can be determined by calculating the percentage of 5mC/(5mC+C)integral area.The results showed that the content of 1-methylhydantoin and cytosine in genome could be determined by HPLC and hemethylation level determined by HPLC was not significant in different regions.

关 键 词：高效液相色谱法 哈蟆油 1-甲基海因 DNA甲基化水平 

分 类 号：O657.72[理学—分析化学]