针刺“调筋”对膝关节骨性关节炎大鼠骨骼肌保护效应的机制研究  

Study on the mechanism of skeletal muscle protection of rats with knee osteoarthritis through regulating tendons with acupuncture

作　　者：富昱 董宝强[1] 林星星[1] 王垒钞 盛泽伟 夏迎春 FU Yu;DONG Bao-qiang;LIN Xing-xing;WANG Lei-hao;SHENG Ze-wei;XIA Ying-chun(School of Acupuncture-Moxibustion and Tuina,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]辽宁中医药大学针灸推拿学院,辽宁沈阳110847

出　　处：《时珍国医国药》2025年第1期169-175,共7页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81574050);辽宁省博士科研启动基金计划项目(2022-BS-196);辽宁省科学技术厅创新能力提升联合基金项目(2022-NLTS-13-04);辽宁省教育厅重点项目(LJKZ0902)。

摘　　要：目的观察针刺结筋病灶点对膝关节骨性关节炎大鼠(KOA)骨骼肌形态、Wnt/β-连环蛋白(Wnt/β-catenin)信号通路及相关炎性因子的影响,探讨经筋刺法治疗KOA的作用机制。方法将40只雄性SD大鼠随机分成正常组、模型组、经穴组、病灶点组,每组10只。除正常组外,其余各组采用膝关节制动法制备KOA模型。经穴组针刺右后肢“犊鼻”“足三里”“阳陵泉”,病灶点组针刺右后肢结筋病灶点,20 min/次,1次/d,共14 d。干预结束后,HE染色法观察大鼠右侧股四头肌形态结构;检测大鼠右侧股四头肌绝对湿重以及计算肌湿重维持率、肌湿重体重比;免疫组织化学染色法检测大鼠右侧股四头肌中MyoD、MyoG以及MyHC1的阳性表达;ELISA法检测大鼠右侧股四头肌中IL-10及TGF-β1含量;Western blot法检测大鼠右侧股四头肌中Wnt、β-catenin蛋白表达水平。结果干预后,与正常组相比,模型组大鼠软骨受损,肌纤维结构破坏,肌绝对湿重、肌湿重维持率、肌湿重体重比降低(P<0.01),MyoD、MyoG阳性表达增加(P<0.01),MyHC1阳性表达降低(P<0.01),IL-10、TGF-β1含量增加(P<0.05,P<0.01),wnt、β-catenin蛋白表达升高(P<0.01,P<0.05);与模型组相比,经穴组大鼠肌绝对湿重、肌湿重维持率、肌湿重体重比增加(P<0.01),MyoD、MyoG、MyHC1阳性表达增加(P<0.01),IL-10、TGF-β1含量增加(P<0.05),Wnt、β-catenin蛋白表达升高(P<0.01,P<0.05);与经穴组相比,病灶点组肌绝对湿重增加(P<0.05),MyoD、MyHC1阳性表达增加(P<0.01,P<0.05),IL-10、TGF-β1含量增加(P<0.01),Wnt、β-catenin蛋白表达升高(P<0.05)。结论针刺结筋病灶点可能通过促进KOA大鼠骨骼肌相关生肌因子表达与抗炎性细胞因子分泌,上调Wnt/β-catenin信号通路相关蛋白表达,调节炎性微环境,进而对骨骼肌起到保护作用。Objective To observe the changes in skeletal muscle morphology,Wnt/β-catenin signaling pathways,and related inflammatory factors in rats with knee osteoarthritis(KOA)through needling the focal points of musculature lesion,and to explore the mechanism of tendon acupuncture in treating KOA.Methods Male SD rats were randomly divided into the normal group,the model group,the lesion group,and the acupoint group,with 10 rats in each.The KOA model was prepared using knee joint immobilization method.Acupuncture in the acupoint group includes the hind limbs“Du Bi”(ST35),“Zu San Li”(ST36),and“Yang Ling Quan”(GB34).Acupuncture was performed on the focal points of musculature lesion in the right hind limb in the lesion group,20min/time,once a day,for a total of 14 days.HE staining method was used to observe the morphological structure of skeletal muscles in each group of rats;muscle mass related indicators of the right quadriceps muscle in rats was detected;immunohistochemical staining was used to detect the positive expressions of MyoD,MyoG,and MyHC1 in the right quadriceps femoris of rats;ELISA method was used to detect the content of IL-10 and TGF-β1 in the right quadriceps muscle of rats.Western blot(WB)was used to detect the protein expressions of Wnt andβ-Catenin in the right quadriceps femoris muscle of rats.Results After intervention,compared with the normal group,the model group rats showed cartilage damage and muscle fiber structure destruction,a decrease in absolute wet weight,maintenance rate of wet weight,and wet weight to body weight ratio(P<0.01),an increase in myogenic factors MyoD and MyoG expressions(P<0.01),MyHC1 expression(P<0.01)was decreased,IL-10,and TGF-β1 were increased(P<0.05,P<0.01),the expression of Wnt andβ-catenin proteins were increased(P<0.01,P<0.05);compared with the model group,the acupoint group showed an increase in absolute muscle wet weight,maintenance rate of muscle wet weight,and muscle wet weight to body weight ratio(P<0.01),as well as an increase in the expression of

关 键 词：膝关节骨性关节炎 经筋 针刺 生肌因子 炎性因子 WNT/Β-CATENIN 

分 类 号：R2-03[医药卫生—中医学]