氟斑牙患儿血清RANK、RANKL、OPG表达水平  

Expression levels of serum RANK,RANKL,and OPG in children with dental fluorosis

作　　者：罗兰英[1] 廖敏[1] 陆皓泉[1] 王芬芬 宁锐军[1] Luo Lanying;Liao Min;Lu Haoquan;Wang Fenfen;Ning Ruijun(Institute of Environmental Health and Endemic Disease Control,Guangxi Zhuang Autonomous Regional Center for Disease Prevention and Control,Nanning 530028,China)

机构地区：[1]广西壮族自治区疾病预防控制中心环境卫生与地方病防制所,南宁530028

出　　处：《中华地方病学杂志》2025年第1期6-9,共4页Chinese Journal of Endemiology

基　　金：广西医疗卫生适宜投术开发与推广应用项目(S201681)。

摘　　要：目的探讨氟斑牙患儿血清核因子κB受体活化因子(RANK)、RANK配体(RANKL)、骨保护素(OPG)的表达水平。方法2018年4月,采用病例-对照研究方法,根据《氟斑牙诊断》(WS/T 208—2011)标准,选择广西壮族自治区一铝冶炼基地周边(1.5 km以内)诊断为氟斑牙的8~12岁儿童作为氟斑牙组(n=49),选择距离该铝冶炼基地5.0 km以上未患氟斑牙的8~12岁健康儿童作为对照组(n=98),采集空腹静脉血,采用离子选择电极法测定血氟含量;双抗体夹心酶联免疫吸附法测定血清RANK、RANKL、OPG表达水平。结果氟斑牙组与对照组血清氟含量[M(Q_(1),Q_(3)):0.033(0.032,0.036)比0.026(0.025,0.028)mg/L]、RANK[1.21(0.87,1.64)比0.73(0.50,1.13)μg/L]、RANKL[81.3(50.6,118.6)比134.3(98.1,199.2)ng/L]、OPG表达水平[433.3(321.6,574.3)比509.1(406.8,709.3)ng/L]和OPG/RANKL比值[5.6(3.1,7.8)比3.6(2.9,5.0)]比较,差异均有统计学意义(Z=8.35、3.83、3.99、2.35、2.47,均P<0.05);相关性分析结果显示,氟斑牙分度与血清氟含量呈正相关(r_(s)=0.68,P<0.001),血清氟含量与血清RANKL、OPG表达水平均呈负相关(r_(s)=-0.49、-0.17,均P<0.05)。结论氟斑牙患儿血清RANK表达水平高于健康儿童,RANKL及OPG表达水平均低于健康儿童。Objective To investigate the expression levels of serum receptor activator of nuclear factor-κB(RANK),RANK ligand(RANKL),and osteoprotegerin(OPG)in children with dental fluorosis.Methods In April 2018,a case-control study was conducted to select 8-12 year old children diagnosed with dental fluorosis residing within 1.5 km of an aluminum smelting facility in Guangxi Zhuang Autonomous Region as the dental fluorosis group(n=49)according to the criteria of"Diagnosis of Dental Fluorosis"(WS/T 208-2011).The control group(n=98)comprised healthy children aged 8-12 years old without dental fluorosis lived more than 5.0 km from the aluminum smelting facility.Fasting venous blood samples were collected from all participants.Blood fluoride level were measured using an ion-selective electrode method.Serum RANK,RANKL,and OPG levels were determined using double-antibody sandwich enzyme-linked immunosorbent assay.Results The levels of serum fluoride[M(Q_(1),Q_(3)):0.033(0.032,0.036)vs 0.026(0.025,0.028)mg/L],RANK[1.21(0.87,1.64)vs 0.73(0.50,1.13)μg/L],RANKL[81.3(50.6,118.6)vs 134.3(98.1,199.2)ng/L],OPG[433.3(321.6,574.3)vs 509.1(406.8,709.3)μg/L],and OPG/RANKL ratio[5.6(3.1,7.8)vs 3.6(2.9,5.0)]were compared between the fluorosis group and the control group,and the differences were statistically significant(Z=8.35,3.83,3.99,2.35,2.47,P<0.05).Correlation analysis revealed that dental fluorosis severity was positively correlated with serum fluoride level(r_(s)=0.68,P<0.001).Serum fluoride level was negatively correlated with both serum RANKL and OPG levels(r_(s)=-0.49,-0.17,P<0.05).Conclusion The level of serum RANK in children with dental fluorosis is higher than that in healthy children,while the levels of RANKL and OPG are lower than those in healthy children.

关 键 词：氟斑牙 核因子ΚB受体活化因子 核因子ΚB受体活化因子配体 骨保护素 

分 类 号：R58[医药卫生—内分泌]