离子选择电极法与高通量快速测定法测定生活饮用水氟含量的比较分析  

Comparative analysis of ion-selective electrode method and high-throughput rapid determination method for determination of fluoride level in drinking water

作　　者：蒲光兰[1] 喇翠玲[1] 鲁青[1] 周昕[1] 陈萍[1] 李亚楠[1] 杨佩珍[1] 胡兰盛[1] 王明君 杨萍[1] 孟献亚[1] 张强[1] Pu Guanglan;La Cuiling;Lu Qing;Zhou Xin;Chen Ping;Li Yanan;Yang Peizhen;Hu Lansheng;Wang Mingjun;Yang Ping;Meng Xianya;Zhang Qiang(Department for Endemic Disease Prevention and Control,Qinghai Provincial Institute for Endemic Disease Prevention and Control,Xining 811602,China)

机构地区：[1]青海省地方病预防控制所地方病预防控制科,西宁811602

出　　处：《中华地方病学杂志》2025年第1期57-60,共4页Chinese Journal of Endemiology

基　　金：第二次青藏高原综合科学考察研究(2019QZKK0607)。

摘　　要：目的分析离子选择电极法和高通量快速测定法测定生活饮用水氟含量的差异。方法采用2种方法测定2种氟化物标准物质、2021—2023年外质控考核水样(每年2种)及3份生活饮用水氟含量(各检测5次)进行精密度检验。测定外质控考核水样及生活饮用水加标回收率进行准确度检验,并根据《生活饮用水卫生标准》(GB 5749—85)进行水样分组(水氟≤1.00、>1.00 mg/L)分析。采用SPSS 23.0软件对测定结果进行统计分析。结果(1)2种方法分别在0.20~5.00、0.000~2.000 mg/L范围内工作曲线相关系数(r)均>0.990,均符合质控要求。(2)精密度检验,2种方法检测2种氟化物标准物质结果比较,差异无统计学意义(F=0.36、0.15,P=0.564、0.707),变异系数(CV)均<5%;外质控考核水样及生活饮用水水样检测结果CV均<5%。(3)准确度检验,水氟≤1.00 mg/L时,2种方法加标回收率比较,差异无统计学意义(F=0.49,P=0.504);水氟>1.00 mg/L时,2种方法加标回收率比较,差异有统计学意义(F=24.75,P=0.003)。结论离子选择电极法具有检测范围宽、适应性较广的优点,而高通量快速测定法准确性较高。检测人员可依据实验室实际条件、样品浓度范围,权衡选择适宜的测定方法。Objective To analyze the differences in determination of fluoride level in drinking water by ion-selective electrode method and high-throughput rapid determination method.Methods The precision test was carried out by using the two methods to measure two kinds of fluoride standard substances,water samples of external quality control assessment from 2021 to 2023(two kinds each year)and the fluoride level in three drinking water samples(for 5 times/each sample).Accuracy testing was conducted by measuring the external quality control assessment water samples and the spiked recovery rates drinking water,and water samples were grouped(water fluoride≤1.00,>1.00 mg/L)and analyzed according to the"Hygienic Standards for Drinking Water"(GB 5749-85).SPSS 23.0 software was used for statistical analysis of the measurement results.Results(1)The correlation coefficients(r)of the working curves of the two methods were both>0.990,meeting the quality control requirements.(2)In the precision test,when comparing the results of the two methods for detecting two kinds of fluoride standard substances,there was no statistically significant difference(F=0.36,0.15,P=0.564,0.707),and the coefficients of variation(CV)were all<5%.The CV of the detection results of the external quality control assessment water samples and drinking water samples were<5%.(3)In the accuracy test,when the fluoride concentration in water was≤1.00 mg/L,there was no statistically significant difference in the spiked recovery rates between the two methods(F=0.49,P=0.504).When the fluoride concentration in water was>1.00 mg/L,there was a statistically significant difference in the spiked recovery rates between the two methods(F=24.75,P=0.003).Conclusions The ion-selective electrode method has the advantages of wide detection range and wide adaptability,while the high-throughput rapid determination method has high accuracy.Testing personnel can weigh and choose the appropriate determination method based on the actual laboratory conditions and sample concentration r

关 键 词：方法 氟化物 离子选择电极法 高通量快速测定法 

分 类 号：R123.1[医药卫生—环境卫生学]