基于网络药理学和生物信息学的化湿宣肺颗粒治疗溃疡性结肠炎机制研究  

Mechanism Study of Huashi Xuanfei Granules in the Treatment of Ulcerative Colitis Based on Network Pharmacology and Bioinformatics

作　　者：郑梅霞 黄飞华 朱婉萍[2,3] ZHENG Meixia;HUANG Feihua;ZHU Wanping(College of Pharmacy,Hangzhou Medical College,Hangzhou,231005;Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine for Pharmacodynamic Material Basis Research of Chinese Medicine,Hangzhou,311305;Tongde Hospital of Zhejiang Province,Hangzhou,310012)

机构地区：[1]杭州医学院药学院,浙江杭州310059 [2]浙江省中药药效物质基础研究中医药重点实验室,浙江杭州311305 [3]浙江省立同德医院,浙江杭州310012

出　　处：《浙江中医杂志》2025年第3期189-193,共5页Zhejiang Journal of Traditional Chinese Medicine

基　　金：浙江省科技计划项目(2021C03033)。

摘　　要：目的:通过网络药理学和生物信息学方法探讨化湿宣肺颗粒(HSXF)治疗溃疡性结肠炎(UC)的关键靶点及潜在药理机制。方法:获取HSXF和UC相关数据,使用Cytoscape软件筛选核心基因,并利用R语言进行基因本体论(GO)富集、京都基因和基因组百科全书(KEGG)通路富集、免疫浸润分析及模型诊断,探讨HSXF治疗UC的潜力与机制。结果:共筛选出669个HSXF相关基因和800个UC差异基因。GO和KEGG分析显示主要富集于炎症和免疫相关生物过程与通路。免疫浸润分析表明,HSXF可靶向调节T细胞和巨噬细胞以治疗UC。模型诊断结果显示核心基因具有较高的诊断价值。实时荧光定量聚合酶链式反应(qPCR)结果证实HSXF能够抑制白细胞介素1β(IL1β)、基质金属蛋白酶1(MMP1)、基质金属蛋白酶9(MMP9)和前列腺素内过氧化物合酶2(PTGS2)的表达。结论:HSXF可通过调控相关信号通路及免疫细胞,靶向IL1β、MMP9、MMP2和PTGS2,发挥对UC的治疗作用。Objective:To investigate the key targets and potential pharmacological mechanisms of Huashi Xuanfei Granules(HSXF)in the treatment of ulcerative colitis(UC)through network pharmacology and bioinformatics methods.Methods:HSXF and UC related data were obtained,the core genes were screened by Cytoscape software,and GO enrichment,KEGG pathway,immune infiltration analysis and model diagnosis were performed by R language,to explore the potential and mechanism of HSXF in the treatment of UC.Results:669 HSXF related genes and 800 UC differential genes were screened.GO and KEGG analysis showed it was mainly enriched in inflammation and immune related biological processes and pathways.Immune infiltration analysis showed that HSXF could target T cells and macrophages to treat UC.The diagnostic results of the model showed that the core gene had a high diagnostic value.The qPCR results confirmed that HSXF could inhibit the expression of IL1β,MMP1,MMP9 and PTGS2.Conclusion:HSXF can play a therapeutic role in treating UC by regulating related signaling pathways and immune cells,targeting IL1β,MMP9,MMP2 and PTGS2.

关 键 词：化湿宣肺颗粒 溃疡性结肠炎 网络药理学 生物信息学 

分 类 号：R285[医药卫生—中药学]