利用高效液相色谱指纹图谱确定马蹄金产地  

Determination of the origin of Dichondra utilize by high performance liquid chromatography fingerprint

作　　者：郭海平 杨红梅[2] 马丽 周维维 王志轩 王世权 董建锋 GUO Haiping;YANG Hongmei;MA Li;ZHOU Weiwei;WANG Zhixuan;WANG Shiquan;DONG Jianfeng(Chengde Food and Drug Inspection Institute,Yanshan Genuine Medicinal Materials in Hebei Province Inspection and Test Technology Innovation Center,Chengde 067000,China;Chengde Nursing Vocational College,Chengde 067002,China)

机构地区：[1]河北省承德市食品药品检验检测中心,河北省燕山道地药材检验检测创新中心,河北承德067000 [2]承德护理职业学院,河北承德067002

出　　处：《化学分析计量》2025年第2期54-59,共6页Chemical Analysis And Meterage

基　　金：河北省药品监督管理局科技计划项目(2022ZC1007)。

摘　　要：采用高效液相色谱法建立10批次不同产地马蹄金指纹图谱,并进行聚类分析。称取0.5 g马蹄金,以70%乙醇为溶剂,加热回流提取样品溶液,使用CACELLPAK-C_(18)色谱柱,流动相为乙腈-0.2%(体积比)磷酸水溶液,梯度洗脱,流量为1.0 m L/min,检测器波长为254 nm,建立马蹄金指纹图谱。10批次马蹄金相似度为0.97~0.99,相对保留时间测定结果的相对标准偏差(RSD)小于1.0%。通过共有模式筛选出8个共有特征峰,其中指认主要1个共有峰为芦丁。方法的重复性、稳定性测试的色谱峰面积RSD均小于1.0%,芦丁的样品加标回收率为98.78%~101.56%,RSD为1.0%(n=6),符合指纹图谱检测要求。运用SPSS 19.0软件进行聚类分析,马蹄金样品分为两大类。该方法具有良好的重复性和稳定性,结合相似度评价和聚类分析,可以评价不同产地马蹄金质量,为马蹄金的质量控制提供参考及对其产地的初步鉴别。The fingerprints of 10 batches of Dichondra repens from different habitats were established by high performance liquid chromatography,and cluster analysis was carried out.A total of 0.5 g of Dichondra repens was weighed,and the sample solution was extracted by heating reflux with 70%ethanol.The chromatographic column was CACELL PAK-C_(18),and the mobile phase was acetonitrile-0.2%(volume ratio)phosphoric acid aqueous solution with gradient elution.The flow rate was 1.0 mL/min,and the detector wavelength was 254 nm to establish the fingerprint of Dichondra repens.The similarities of 10 batches of dichondra were between 0.97 to 0.99,and the relative standard deviation of relative retention time test results was less than 1.0%.Eight common characteristic peaks were screened out by the common mode,and one of the main common peaks was identified as rutin.The RSD of the chromatographic peak area of the repeatability and stability test of the method was less than 1.0%.The recovery rate of rutin was 98.78%-101.56%,and the RSD was 1.0%(n=6),which met the requirements of fingerprint detection.Cluster analysis was performed by using SPSS 19.0 software,and the samples were divided into two categories.The method has good repeatability and stability.Combined with similarity evaluation and cluster analysis,the quality of Dichondra repens from different habitats can be evaluated,which can provide reference for the quality control of Dichondra repens and the preliminary identification of its origin.

关 键 词：马蹄金 高效液相色谱 指纹图谱 聚类分析 

分 类 号：O657.7[理学—分析化学]