高效液相色谱法联合化学模式识别分析不同产地荷叶中生物碱类成分差异  

Analysis of the differences in alkaloid components in Nelumbinis Folium from different regions using high performance liquid chromatography combined with chemical pattern recognition

作　　者：杨乐怡 林天凤 黄蓉 蔡园 周昶 李煜苗 张颜 于密密 刘斌[1,3] 姜艳艳 YANG Leyi;LIN Tianfeng;HUANG Rong;CAI Yuan;ZHOU Chang;LI Yimiao;ZHANG Yan;YU Mimi;LIU Bin;JIANG Yanyan(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102401,China;Beijing Institute for Drug Control,NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine,Beijing 102206,China;The Key Research Laboratory of"Exploring Effective Substance in Classic and Famous Prescriptions of Traditional Chinese Medicine",The State Administration of Traditional Chinese Medicine of the People's Republic of China,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102401 [2]北京市药品检验研究院,国家药品监督管理局中药(中成药)质量评价重点实验室,北京市中药分析评价重点实验室,北京102206 [3]中国中医药管理局“探索中药经典名方有效成分”重点研究实验室,北京102488

出　　处：《化学分析计量》2025年第2期99-107,共9页Chemical Analysis And Meterage

摘　　要：采用高效液相色谱指纹图谱及化学模式识别比较不同产地荷叶中生物碱类成分的差异性,筛选出生物碱类差异性标志物,建立了荷叶生物碱类成分含量测定方法。荷叶指纹图谱中确定了24个共有峰,指认了8个生物碱类成分;聚类分析和主成分分析结果表明同一产地荷叶的质量较接近;正交偏最小二乘法-判别分析结果发现,有5种生物碱类成分是造成不同产地间荷叶质量差异的标志物;含量测定结果显示,大部分内陆省市的荷叶中生物碱类标志物含量较高。该方法可反映不同产地荷叶的质量差异,为荷叶的质量评价及控制提供参考。The differences was compared in alkaloid components in different origins of Nelumbinis Folium using high performance liquid chromatography fingerprint chromatogram,and chemical pattern recognition Alkaloid-type differential markers were screened out and a method for determining the content of alkaloid components in Nelumbinis Folium was established.A total of 24 common peaks were identified in the lotus leaf fingerprint chromatogram,and 8 alkaloid components were identified.Hierarchical cluster analysis and principal component analysis results showed that the quality of Nelumbinis Folium from the same origin was relatively close.Orthogonal partial least squares discriminant analysis results showed that there were 5 alkaloid components that were differential markers between different origins of Nelumbinis Folium.The content determination results showed that the content of alkaloid markers in most inland provinces was higher.This method can reflect the quality differences between different origins of Nelumbinis Folium and provide reference for the quality evaluation and control of Nelumbinis Folium.

关 键 词：荷叶 生物碱 高效液相色谱法 指纹图谱 化学模式识别 

分 类 号：O657.7[理学—分析化学]