羟基磷灰石-蟛蜞菊内酯联合促成骨分化作用  

Effect of hydroxyapatite and wedelolactone on osteogenic differentiation

作　　者：朱迪 陈光明 刘艳秋 ZHU Di;CHEN Guangming;LIU Yanqiu(Department of General Medicine,Affiliated Jinhua Hospital,Zhejiang University School of Medicine,Jinhua 321000,Zhejiang,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China)

机构地区：[1]浙江大学医学院附属金华医院全科医学科,浙江金华321000 [2]山东中医药大学中医学院,山东济南250355

出　　处：《中国现代医生》2025年第5期1-4,共4页China Modern Doctor

基　　金：国家自然科学基金项目(81473545);浙江省金华市重大(重点)科学技术研究计划项目(2021-3-055)。

摘　　要：目的探讨羟基磷灰石-蟛蜞菊内酯(hydroxyapatite-wedelolactone,Hap-Wed)联合的体外释药行为及促骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化作用。方法利用色谱技术检测Hap-Wed融合材料体外释药行为。细胞连续传至第3代后分为:①对照组:完全培养基;②Os组:完全培养基中加入成骨诱导剂(1mmol/L地塞米松、10mmol/L维生素C、1mol/Lβ-甘油磷酸钠);③Wed组:完全培养基中加入1μg/ml Wed和成骨诱导剂;④Hap-Wed组:完全培养基中加入1μg/ml Wed、成骨诱导剂及Hap。采用碱性磷酸酶(alkaline phosphates,ALP)活性实验、茜素红S染色实验、实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测骨钙素、Osterix和Runx2的基因表达情况,分析Hap-Wed对BMSCs成骨细胞分化的影响。结果Hap-Wed融合材料可缓慢释放药物,Hap-Wed组与对照组比较,ALP染色阳性数量、钙化结节数量均显著增加。Hap-Wed组骨钙素、Osterix和Runx2的基因表达提高。结论Hap-Wed融合材料可缓慢、有效刺激成骨细胞分化,可为今后蟛蜞菊内酯的载体材料发展提供很好的应用前景。Objective To investigate the in vitro drug release behavior of hydroxyapatite-wedelolactone(Hap-Wed)and its effect on promoting osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods The in vitro release behavior of Hap-Wed fusion materials was detected by chromatographic technique.After the cells were passed continuously up to the third generation,they were divided into:①control group:complete medium;②Os group:osteogenic inducer(1mmol/L dexamethasone,10mmol/L vitamin C,1mol/L β-glycerol phosphate);③Wed group:1μg/ml Web and osteogenic inducer were added to the complete medium;④Hap-Web group:1μg/ml Web,osteogenic inducer and Hap were added to the complete medium.Alkaline phosphates(ALP)activity assay,alizarin red S staining assay,real-time quantitative polymerase chain reaction(RT-qPCR)detection of osteocalcin,Osterix and Runx2 gene expression,analysis of Hap-Wed on BMSCs osteoblast differentiation.Results Hap-Wed fusion material released the drug slowly.Compared with control group,the number of ALP staining and the number of calcified nodules increased significantly in Hap-Wed experimental group.The Hap-Wed experimental group increased gene expression of osteocalcin,Osterix and Runx2.Conclusion Hap-Wed fusion material can slow and effective stimulation of osteoblast differentiation,which may provide a promising application prospect for the future development of the carrier material.

关 键 词：羟基磷灰石 蟛蜞菊内酯 成骨分化 骨质疏松症 

分 类 号：R285.5[医药卫生—中药学]