基于网络药理学探讨芍甘散镇痛作用机制研究  

Study on the analgesic mechanism of Shaogan San based on network pharmacology

作　　者：魏志勇 苏源冰 WEI Zhiyong;SU Yuanbing(SectionⅡ,Department of Lower Extremity,Quanzhou Orthopedic-Traumatological Hospital,Fujian Province,Quanzhou 362000,China)

机构地区：[1]福建省泉州市正骨医院下肢二科,福建泉州362000

出　　处：《中国当代医药》2025年第4期4-8,共5页China Modern Medicine

摘　　要：目的基于网络药理学研究方法探讨经验方芍甘散镇痛的作用机制。方法通过TCMSP数据库搜索芍甘散有效成分及潜在靶点;通过GeneCards数据库收集疼痛的疾病靶点,并获取药物与疾病的交集靶点;采用Cytoscape3.9.1构建药物-成分-交集靶点网络图,筛选出核心成分;采用String数据库,绘制构建蛋白质相互作用网络图,筛选出核心靶点;使用David数据库进行GO功能富集分析及KEGG通路富集分析。结果芍甘散有效成分167个,其潜在靶点与疼痛靶点的交集有224个,“药物-成分-靶点-疾病”网络提示芍甘散的核心化合物为槲皮素、山柰酚、7-甲氧基-2-甲基异黄酮、β-谷甾醇、柚皮素、异鼠李素、芒柄花黄素、美迪紫檀素、甘草查尔酮A、豆甾醇;“药物-成分-靶点-疾病”网络结合蛋白质相互作用网络提示雌激素受体、COX2、热休克蛋白90、钙调蛋白受体、蛋白激酶、白细胞介素-6、抑癌蛋白、白介素-1β可能是关键靶点。KEGG通路富集分析显示核心通路主要包括癌症的信号通路、脂质与动脉粥样硬化的信号通路、糖尿病并发症中的AGE-RAGE信号通路、流体剪切应力与动脉粥样硬化信号通路等。结论芍甘散可能通过多靶点、多信号途径的相互作用起到镇痛作用,可为下一步实验研究提供依据。Objective To explore the analgesic mechanism of Shougan San based on network pharmacology research methods.Methods The TCMSP database was used to search for effective components and potential targets of Shaogan San,the GeneCards database was used to collect targets of pain diseases and obtain intersection targets with drugs.Cytoscape 3.9.1 was used to construct a drug-component-intersection target network diagram,selecting core components.String database was used to draw protein interaction network diagrams and select core targets.David database was used for GO functional enrichment analysis and KEGG pathway enrichment analysis.Results There are 167 effective components in Shaogan San,with 224 intersection between potential targets and pain targets.The"drug-component-target-disease"network suggests that the core compounds of Shaogan San are quercetin,kaempferol,7-methoxy-2-methyl isoflavones,β-sitosterol,naringenin,isorhamneti,formononetin,Medicarpin,licochalcone A,stigmasterol.The"drug-component-target-disease"network combined with the protein interaction network indicates that estrogen receptor,COX2,heat shock protein 90,calmodulin,protein kinase,interleukin-6,cancer suppressor protein,interleukin-1β may be key targets.KEGG pathway enrichment analysis showed that the core pathways mainly included cancer signaling pathway,lipid and atherosclerosis signaling pathway,AGE-RAGE signaling pathway in diabetes complications,fluid shear stress and atherosclerosis signaling pathway.Conclusion Shaogan San may play an analgesic role through the interaction of multi-target and multi-signal pathways,which can provide a basis for further experimental research.

关 键 词：网络药理学 芍甘散 镇痛 经验方 

分 类 号：R289[医药卫生—方剂学]