毛蕊花糖苷对人增生性瘢痕成纤维细胞的干预及机制研究  

Intervention and mechanism study of verbascoside on human hypertrophic scar fibroblasts

作　　者：卜盼盼 齐郁松 赵皎均 马少林[1] BU Panpan;QI Yusong;ZHAO Jiaojun;MA Shaolin(Department of Plastic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830011,China)

机构地区：[1]新疆医科大学第一附属医院整形外科,新疆乌鲁木齐830011

出　　处：《中国医药导报》2025年第4期7-12,43,共7页China Medical Herald

基　　金：国家自然科学基金资助项目(81760345)。

摘　　要：目的基于TGF-β1/Smads信号通路探讨毛蕊花糖苷对人增生性瘢痕成纤维细胞的作用及其机制研究。方法CCK8法检测毛蕊花糖苷对增生性瘢痕成纤维细胞的半数抑制浓度。将实验分为对照组、毛蕊花糖苷低浓度组(40μg/ml毛蕊花糖苷)、毛蕊花糖苷组高浓度(80μg/ml毛蕊花糖苷)。划痕实验检测不同浓度毛蕊花糖苷对增生性瘢痕成纤维细胞的迁移能力的影响,流式细胞术检测不同浓度毛蕊花糖苷对增生性瘢痕成纤维细胞的细胞周期与凋亡的影响。选用最佳浓度的TGF-β1作为模型组,再将实验分为对照组、模型组:5 ng/ml TGF-β1;低浓度实验组(TGF-β1+40μg/ml毛蕊花糖苷);高浓度实验组(TGF-β1+80μg/ml毛蕊花糖苷)。实时荧光定量PCR、Western blot法检测四组Smad2、Smad3和COL-1的mRNA及蛋白表达变化。结果毛蕊花糖苷对增生性瘢痕成纤维细胞的半数抑制浓度为79.54μg/ml。毛蕊花糖苷低、高浓度组增生性瘢痕成纤维细胞迁移距离小于对照组(P<0.05)。与对照组比较,毛蕊花糖苷低、高浓度组均能将增生性瘢痕成纤维细胞的细胞增殖周期阻滞在G0/G1期(P<0.05)。与对照组比较,毛蕊花糖苷高浓度组成纤维细胞凋亡率升高(P<0.01)。与对照组比较,模型组中Smad2、Smad3及COL-1 mRNA的表达量升高(P<0.05);与模型组比较,低、高浓度实验组Smad2、Smad3及COL-1 mRNA表达量降低(P<0.05)。与对照组比较,模型组P-Smad2/3、COL-1蛋白表达水平升高(P<0.05);与模型组比较,高浓度实验组P-Smad2/3表达水平降低,低、高浓度实验组COL-1蛋白表达水平降低(P<0.05)。结论毛蕊花糖苷可通过抑制TGF-β1/Smads信号通路进而抑制增生性瘢痕成纤维细胞的活化和增殖。Objective To explore the role of verbascoside on human hypertrophic scar fibroblasts and its mechanism based on TGF-β1/Smads signaling pathway.Methods The half-inhibitory concentration of verlein on hypertrophic scar fibroblasts was determined by CCK8 method.The experiment was divided into control group and low concentration group(40μg/ml uerbascoside),high concentration of verminin group(80μg/ml verbascoside).The effects of different concentrations of uerbascoside on the migration ability of fibroblasts in hypertrophic scar were detected by scratch test,and the changes of cell cycle and apoptosis of fibroblasts in hypertrophic scar were detected by flow cytometry.The optimal concentration of TGF-β1 was selected as the model group,and then the experiment was divided into control group and model group:5 ng/ml TGF-β1;low concentration experimental group(TGF-β1+40μg/ml verbascoside);high concentration experimental group(TGF-β1+80μg/ml verbascoside).The mRNA and protein expression of Smad2,Smad3,and COL-1 in the four groups were detected by real-time fluorescence quantitative PCR and Western blot.Results The median inhibitory concentration of verlein on hypertrophic scar fibroblasts was 79.54μg/ml.The migration distance of hypertrophic scar fibroblasts in low and high concentration of verbascoside was smaller than that in control group(P<0.05).Compared with the control group,the cell proliferation cycle of hypertrophic scar fibroblasts could be blocked in G0/G1 phase in both low and high concentration of vervascoside(P<0.05).Compared with the control group,the apoptosis rate of fiber cells with high concentration of verbascoside was increased(P<0.01).Compared with control group,mRNA expressions of Smad2,Smad3,and COL-1 in model group were increased(P<0.05).Compared with model group,mRNA expressions of Smad2,Smad3,and COL-1 in low and high concentration groups were decreased(P<0.05).Compared with control group,the expression levels of P-SMAD2/3 and COL-1 protein in model group were increased(P<0.05).Compared

关 键 词：增生性瘢痕成纤维细胞 TGF-β1/Smads信号通路 毛蕊花糖苷 

分 类 号：R62[医药卫生—整形外科]