五子衍宗丸改善急进高原生殖损伤的机制研究  

Study on the mechanism of Wuzi Yanzong Pills to improve reproductive injury in rapid plateau

作　　者：王世尚 李倩 杨峰[2] 范宁 李萌 王瑞[3] 王晓锋 尹东锋[1] WANG Shishang;LI Qian;YANG Feng;FAN Ning;LI Meng;WANG Rui;WANG Xiaofeng;YIN Dongfeng(School of Pharmacy,Shihezi University,Xinjiang Uygur Autonomous Region,Shihezi 832003,China;Department of Pharmacy,General Hospital of Xinjiang Military Command,Xinjiang Uygur Autonomous Region,Urumqi 830000,China;Reproductive Medicine Center,General Hospital of Xinjiang Military Command,Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]石河子大学药学院,新疆石河子832003 [2]新疆军区总医院药剂科,新疆乌鲁木齐830000 [3]新疆军区总医院生殖医学中心,新疆乌鲁木齐830000

出　　处：《中国医药导报》2025年第4期20-26,共7页China Medical Herald

基　　金：新疆维吾尔自治区重点研发计划项目(2022B 03005-3)。

摘　　要：目的基于转录组测序技术和实验验证探究五子衍宗丸对急进高原雄性生殖损伤的保护机制。方法6周龄SD大鼠72只,采用随机数字法将大鼠分为对照组、模型组、给药组,每组24只,每组再分为1、3、5 d 3个时间段,每个时间段8只。建立急进高原雄性SD大鼠生殖损伤模型,采用灌胃给药的方式进行干预,给药组给予五子衍宗丸1.0 g/kg,对照组和模型组给予同等剂量的0.9%NaCl溶液,1次/d,采取灌胃给药的方式,直到达到对应1、3、5 d后进行解剖取材。对正常组、模型组与五子衍宗丸效果最好的时间段大鼠睾丸进行转录组测序,采用生物信息学分析方法筛选出差异表达基因,随后基于差异表达基因构建蛋白-蛋白相互作用网络分析筛选核心基因,并且通过定量聚合酶链式反应进行验证。最后,通过通路富集分析,探究五子衍宗丸改善急性低压低氧生殖损伤的关键信号通路。结果3个时间段模型组大鼠精子数目、睾酮低于对照组(P<0.05);给药组精子数目、睾酮高于模型组(P<0.05)。基于蛋白-蛋白相互作用网络的核心基因分析共得到5个候选核心基因:Pcdhb1、Cxcl10、Irf7、Mx1、Ifi44。随后通过定量聚合酶链式反应发现Pcdhb1是五子衍生丸改善急进高原雄性生殖损伤的核心基因。与对照组比较,模型组Pcdhb1基因表达量下降;与模型组比较,给药组Pcdhb1基因表达量升高(P<0.05)。通过通路富集分析发现,五子衍宗丸治疗急进高原生殖损伤关键信号通路是Wnt信号通路。结论五子衍宗丸通过上调Pcdhb1基因的表达通过Wnt信号通路改善急进高原雄性生殖损伤,为后续五子衍生丸的大规模临床应用提供数据支持。Objective To explore the protective mechanisms of Wuzi Yanzong Pills against acute high-altitude male reproductive injury based on transcriptome sequencing technology and experimental verification.Methods Seventy-two 6-week-old SD rats were divided into control group,model group,and treatment group by random number table method,with 24 rats in each group,each group was further divided into three time periods of 1,3,and 5 days,with 8 rats in each period.Acute high-altitude male SD rat reproductive damage model was established,and intervention was conducted through gavage administration,the treatment group received Wuzi Yanzong Pills at a dosage of 1.0 g/kg and the control and model group received equivalent volume of 0.9%NaCl solution,once daily,until the respective days were reached.Transcriptome sequencing was performed on the control grgoup,model group,and the best-responding periods to Wuzi Yanzong Pills.Bioinformatics analysis methods were used to screen for differentially expressed genes,and a proteinprotein interaction network was constructed based on these genes to identify core genes.Quantitative polymerase chain reaction was then used for validation.Finally,pathway enrichment analysis was conducted to investigate the key signaling pathways through which Wuzi Yanzong Pills improved acute hypobaric hypoxia-induced reproductive damage.Results The sperm number and testosterone in the model group were lower than those in the control group (P<0.05). The sperm count and testosterone in the administration group were higher than those in the model group (P<0.05). Core gene analysis based on the proteinprotein interaction network identified five candidate core genes: Pcdhb1, Cxcl10, Irf7, Mx1, and Ifi44. Subsequent quantitative polymerase chain reaction revealed that Pcdhb1 was the core gene through which Wuzi Yanzong Pills improved acute high-altitude male reproductive damage. Compared with the control group, the expression level of Pcdhb1 gene in the model group was decreased;compared with model group, the expre

关 键 词：五子衍宗丸 急进高原 雄性生殖损伤 差异分析 通路富集 

分 类 号：R285.5[医药卫生—中药学]