免疫相关基因对头颈部鳞状细胞癌患者预后的影响  

Effect of immune-related genes in the prognosis of patients with head and neck squamous carcinoma

作　　者：史晨雪 许敏 许雨欣 王剑 邬振华[1] SHI Chenxue;XU Min;XU Yuxin;WANG Jian;WU Zhenhua(Department of Otolaryngology,Head and Neck Surgery,Affiliated Lihuili Hospital of Ningbo University,Ningbo 315000,Zhejiang,China;Department of Neurology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221006,Jiangsu,China;Department of Otolaryngology,Head and Neck Surgery,the Affiliated People’s Hospital of Ningbo University,Ningbo 315040,Zhejiang,China)

机构地区：[1]宁波大学附属李惠利医院耳鼻咽喉头颈外科,浙江宁波315000 [2]徐州医科大学附属医院神经内科,江苏徐州221006 [3]宁波大学附属人民医院耳鼻咽喉头颈外科,浙江宁波315040

出　　处：《中国现代医生》2025年第7期36-42,53,共8页China Modern Doctor

摘　　要：目的通过生物信息学方法对头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)患者的基因表达进行分析,寻找影响HNSCC患者预后的免疫相关基因,探索HNSCC新的治疗靶点。方法本研究基于癌症基因组图谱(The Cancer Genome Atlas,TCGA)和基因表达综合数据库,下载HNSCC和非肿瘤组织样本的RNA测序数据。分别对两数据库样本进行差异表达基因分析和加权基因共表达网络分析,筛选免疫相关基因。采用单因素Cox回归、Lasso回归和多因素Cox回归分析识别出与HNSCC预后显著相关的基因,并以此为基础计算每个TCGA样本的风险评分,构建相关预后模型。根据风险评分中位数将样本分为高、低风险组;对高、低风险组进行基因差异表达分析,揭示不同免疫状态下的基因表达变化,并对差异基因进行通路富集分析。结果筛选发现MS4A1、IL12RB2、DMBT1、LTF是影响HNSCC患者预后的免疫相关基因。其中MS4A1和IL12RB2在HNSCC中高表达,DMBT1和LTF低表达。HNSCC死亡组的风险评分显著高于生存组。风险评分可作为HNSCC的独立预后指标。高、低风险组差异基因显著富集于免疫应答调节细胞表面受体信号通路。结论MS4A1、IL12RB2、DMBT1、LTF可用于HNSCC的风险评估、疾病监测和疗效评估。免疫反应在HNSCC患者的预后中至关重要,进一步的研究有望推动新治疗策略的开发。Objective To analyze the gene expression of patients with head and neck squamous cell carcinoma(HNSCC)using bioinformatics methods,identifying immune-related genes that impact prognosis and exploring new therapeutic targets for HNSCC.Methods This study was based on The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus database to download RNA sequencing data from HNSCC and non-tumor tissue samples.Differential expression gene analysis and weighted gene co-expression network analysis were performed respectively to screen immune-related genes.Univariate Cox regression,Lasso regression and multifactor Cox regression analyses were used to identify the genes significantly associated with HNSCC prognosis,and on this basis,the risk score of each TCGA sample was calculated and the correlation prognosis model was constructed.The sample was divided into high and low risk groups according to the median risk score.Gene differential expression analysis was performed in high and low risk groups to reveal gene expression changes under different immune states,and pathway enrichment analysis was performed for differential genes.Results Screening identified MS4A1,IL12RB2,DMBT1 and LTF as immune-related genes affecting the prognosis of HNSCC.Among them,MS4A1 and IL12RB2 were highly expressed in HNSCC,and DMBT1 and LTF were lowly expressed.Risk score of HNSCC death group was significantly higher than that of the survival group.Risk score could be used as an independent prognostic indicator for HNSCC.Differential genes in high and low risk groups were significantly enriched in immune response-regulating cell surface receptor signaling pathways.Conclusion MS4A1,IL12RB2,DMBT1 and LTF can be used for risk assessment,disease surveillance and efficacy evaluation of HNSCC.The immune response is crucial in the prognosis of HNSCC patients,and further studies are expected to drive the development of new therapeutic strategies.

关 键 词：头颈部鳞状细胞癌 免疫 预后 生物信息学 

分 类 号：R653[医药卫生—外科学] R739.91[医药卫生—临床医学]