复方中药厚朴温中汤镇痛作用效果及代谢机制的研究  

Study on the analgesic effect and metabolic mechanism of compound Chinese medicine Houpu Wenzhong decoction

作　　者：杨代晓 许雄伟 申健 彭晓放 汤莉[2] 张磊 彭拓华 YANG Daixiao;XU Xiongwei;SHEN Jian;PENG Xiaofang;TANG Li;ZHANG Lei;PENG Tuohua(Guangdong Province Institute of Biological Products and Materia Medica,Guangzhou 510440,China;Guangdong Provincial Center for Disease Prevention and Control,Guangzhou 510440,China)

机构地区：[1]广东省生物制品与药物研究所,广州510440 [2]广东省疾病预防控制中心,广州510440

出　　处：《药学前沿》2025年第2期201-209,共9页China Pharmacist

基　　金：广东省医学科学技术研究基金项目(B2023031);广东省中医药局科研项目(20242011);“广东特支计划”卫生健康平台科技创新人才项目(0620220104);广东省科技计划项目(2023B1212010010)。

摘　　要：目的本研究旨在通过气相色谱-质谱联用(GC/MS)代谢组学技术,深入探讨厚朴温中汤的镇痛作用及其潜在的代谢机制,为中药镇痛药物的开发提供科学依据。方法采用热板法和醋酸扭体法评估厚朴温中汤的镇痛效果,将小鼠随机分为模型组、阳性对照组(阿司匹林,0.2 g/kg)和厚朴温中汤高(3.6 g/kg)、中(1.8 g/kg)、低(0.9 g/kg)剂量组,连续给药7 d后,测定小鼠在热板上的舔足潜伏期;将小鼠随机分为空白组、模型组、厚朴温中汤组(3.6 g/kg),连续给药7 d后,计算醋酸诱导的扭体次数。利用GC/MS代谢组学分析小鼠血液样本,筛选潜在生物标志物并解析相关代谢通路。结果热板法实验结果显示,厚朴温中汤具有显著的剂量依赖性镇痛效果,与模型组比较,高、中剂量组在给药后30 min和60 min时舔足潜伏期显著延长(P<0.05);醋酸扭体实验结果显示,厚朴温中汤组小鼠扭体次数较模型组显著降低(P<0.05)。代谢组学分析发现19个潜在生物标志物,涉及7条代谢通路,其中丙氨酸、天冬氨酸和谷氨酸代谢通路尤为关键,丙氨酸的丰度变化与镇痛效果密切相关。结论本研究通过热板法和醋酸扭体法证实厚朴温中汤具有显著的镇痛作用,并基于代谢组学技术揭示其镇痛机制与丙氨酸、天冬氨酸和谷氨酸代谢通路密切相关,为厚朴温中汤的进一步研究及药物开发提供了重要的理论依据。Objective To explore the analgesic effects of Houpu Wenzhong decoction and its underlying metabolic mechanisms using gas chromatography-mass spectrometry(GC/MS)metabolomics technology,and provide scientific evidence for the development of traditional Chinese medicine(TCM)analgesics.Methods The analgesic effects of Houpu Wenzhong decoction were evaluated using the hot-plate test and acetic acid-induced twisting test.Mice were randomly divided into the model group,the positive control group(aspirin,0.2 g/kg),and high(3.6 g/kg),medium(1.8 g/kg),and low(0.9 g/kg)dose groups of Houpu Wenzhong decoction.After continuous administration for 7 days,the latent period of licking feet in mice on a hot plate was measured.Mice were randomly divided into the blank group,the model group,and the Houpu Wenzhong decoction group(3.6 g/kg).After continuous administration for 7 days,the number of twisting induced by acetic acid were calculated.GC/MS metabolomics was used to analyze the blood samples of mice to identify potential biomarkers and elucidate related metabolic pathways.Results The experimental results of the hot plate method showed that Houpu Wenzhong decoction had a significant dose-dependent analgesic effect,and compared with the model group,the high and medium dose groups showed significantly prolonged latency of foot licking in the 30th and 60th minute after administration(P<0.05).The results of the acetic acid twisting experiment showed that the twisting frequency of mice in the Houpu Wenzhong decoction group was significantly reduced compared to the model group(P<0.05).Metabolomics analysis identified 19 potential biomarkers involving 7 metabolic pathways,among which the alanine,aspartate,and glutamate metabolic pathways were particularly crucial,with alanine abundance changes closely related to the analgesic effects.Conclusion This study confirms the significant analgesic effects of Houpu Wenzhong decoction through the hot plate and acetic acid-induced twisting tests,and elucidates its analgesic mechanisms relating t

关 键 词：中药 厚朴温中汤 代谢组学 热板法 醋酸扭体法 代谢通路 镇痛机制 

分 类 号：R285.5[医药卫生—中药学]