基于UPLC特征图谱和2种成分含量测定的皂角刺药材质量评价  

Quality evaluation of Gleditsiae spina based on UPLC specific chromatogram and two components determination

作　　者：位翠杰 刘晓霞 冯涌微 张芳平 段志文 谢文凯 夏天睿 李振雨 何民友 陈向东 孙冬梅 WEI Cuijie;LIU Xiaoxia;FENG Yongwei;ZHANG Fangping;DUAN Zhiwen;XIE Wenkai;XIA Tianrui;LI Zhenyu;HE Minyou;CHEN Xiangdong;SUN Dongmei(Guangdong Yi Fang Pharmaceutical Co.,Ltd.,Foshan 528244,Guangdong Province,China;Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244,Guangdong Province,China)

机构地区：[1]广东一方制药有限公司,广东佛山528244 [2]广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《药学前沿》2025年第2期227-235,共9页China Pharmacist

基　　金：2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台建设(2022-230-221);2022年佛山市南海区重点领域科技攻关专项项目。

摘　　要：目的建立皂角刺药材超高效液相色谱(UPLC)特征图谱及测定东莨菪内酯、花旗松素含量的方法,为全面评价皂角刺药材的质量提供参考。方法采用UPLC法建立了皂角刺药材特征图谱并同时测定东莨菪内酯、花旗松素2种有效成分含量的方法。根据特征图谱和含量测定结果,对17批皂角刺药材进行聚类分析,并对不同产地药材和常见伪品进行对比分析。结果建立皂角刺药材特征图谱,确定8个特征峰,经质谱分析及与对照品比对,确认其中6个特征峰的成分,分别为原儿茶醛、东莨菪苷、东莨菪内酯、花旗松素、牡荆素和异牡荆素;17批皂角刺药材被为两类;河南省洛阳市产地药材含量较高,陕西省和四川省产地药材中花旗松素含量较低;所建立特征图谱和含量测定方法均能对其常见的4种伪品进行快速、有效地区分鉴别。结论该方法为建立完善的皂角刺药材质量标准及全面评价其质量提供参考。Objective To establish ultra performance liquid chromatography(UPLC)specific chromatogram of Gleditsiae spina,determine the contents of scopoletin and taxifolin,and provide reference for comprehensively evaluating the quality of Gleditsiae spina.Methods The UPLC method was used to establish the specific chromatogram of Gleditsiae spina and determine the contents of two active components,scopoletin and taxifolin.According to the specific chromatogram and component determination results,clustering analysis was performed on 17 batches of Gleditsiae spina,and comparative analysis was performed on medicinal materials from different origins and common counterfeits.Results The specific chromatogram of Gleditsiae spina was established,with 8 characteristic peaks identified.After mass spectrometry analysis and comparison with reference substances,it was confirmed that the components of 6 characteristic peaks were protocatechualdehyde,scopolin,scopoletin,taxifolin,vitexin and isovitexin.The 17 batches of Gleditsiae spina were divided into two categories,the content of Gleditsiae spina was higher in Luoyang,Henan province,and the content of taxifolin in Gleditsiae spina was lower in Shaanxi Province and Sichuan Province.The method of characteristic spectrum and content determination can be used to quickly and effectively distinguish the common 4 counterfeits.Conclusion This method can provides reference for establishing a comprehensive quality standard for Gleditsiae spina and comprehensively evaluating its quality.

关 键 词：皂角刺 超高效液相色谱特征图谱 东莨菪内酯 花旗松素 伪品 

分 类 号：R284.1[医药卫生—中药学]