γ-谷氨酰转肽酶驱动双面神纳米马达的制备及其运动行为  

Preparation ofγ-Glutamyl Transferase Driving Janus-structured Nanomotors and Their Motion Behavior

作　　者：裴子叶 邓雨莲 官建国[1,2] 罗明 PEI Ziye;DENG Yulian;GUAN Jianguo;LUO Ming(State Key Laboratory of Advanced Technology for Materials Synthesis and Processing,Wuhan University of Technology,Wuhan 430070,Hubei,China;International School of Materials Science and Engineering(School of Materials and Microelectronics),Wuhan University of Technology,Wuhan 430070,Hubei,China)

机构地区：[1]武汉理工大学材料复合新技术国家重点实验室,湖北武汉430070 [2]武汉理工大学材料科学与工程国际化示范学院(材料与微电子学院),湖北武汉430070

出　　处：《济南大学学报(自然科学版)》2025年第2期299-305,共7页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金项目(22075222)。

摘　　要：为了研究化学驱动微纳米马达的自主运动行为,利用全溶液化学合成法制备γ-谷氨酰转肽酶驱动双面神纳米马达。利用选择性刻蚀和分步偶联法,制备一侧修饰羧基的双面神纳米金颗粒;利用碳二亚胺-N-羟基琥珀酰亚胺偶联技术,将γ-谷氨酰转肽酶选择性修饰在双面神纳米金颗粒一侧制得γ-谷氨酰转肽酶驱动双面神纳米马达,采用透射电子显微镜、场发射扫描电子显微镜和倒置暗场显微镜等表征该纳米马达的结构形貌、驱动性能和趋化性。结果表明:制备的γ-谷氨酰转肽酶驱动双面神纳米马达的直径为(90.0±5.3)nm,分散性良好;该纳米马达在谷胱甘肽水溶液中的运动呈现含量依赖性,当谷胱甘肽的浓度达到10 mmol/L时,扩散系数达到2.39μm^(2)/s;该纳米马达具有良好的离子耐受性,能在不同生物介质中高效驱动,并能高灵敏感知谷胱甘肽浓度梯度产生正趋化性,向谷胱甘肽高含量区域富集。To study the autonomous motion behavior of chemical driving micro-and nano-motors,γ-glutamyl transferase driving Janus-structured nanomotors were prepared by using a colloidal chemical synthesis method.The carboxyl-modified Janus Au nanoparticles were prepared by selective etching and step-by-step coupling method,thenγ-glutamyl transferase was selectively modified on one side of the Janus Au nanoparticles using carbodiimide-N-hydroxysuccinimide coupling method to obtainγ-glutamyl transferase driving Janus-structured nanomotors.Transmission electron microscopy,scanning electron microscopy,and inverted dark-field microscopy were used to characterize the morphology,motion performance,and chemotaxis of the nanomotors.The results show that the prepared nanomotors have a diameter of(90.0±5.3)nm with good dispersibility.The nanomotors exhibit content-dependent motion performance in glutathione aqueous solutions,when the concentration of glutathione is 10 mmol/L,their diffusion coefficient reaches 2.39μm^(2)/s.The nanomotors not only display good ionic tolerance and can efficiently drive in different biological media,but also can sense the glutathione concentration gradient to exhibit positive chemotaxis and enrich in the area with high glutathione content.

关 键 词：纳米马达 双面神结构 Γ-谷氨酰转肽酶 谷胱甘肽 趋化作用 

分 类 号：TB381[一般工业技术—材料科学与工程]