MAGI2-AS3通过负向调控miR-934促进BTN3A3表达而抑制神经内分泌肿瘤细胞的增殖、迁移和侵袭并促进凋亡  

MAGI2-AS3 inhibits the proliferation,migration,and invasion and promotes apoptosis of neuroendocrine tumor cells by negatively regulating miR-934 to enhance BTN3A3 expression

作　　者：蒋君霞 唐澍[1] 喻珣 Jiang Junxia;Tang Shu;Yu Xun(Department of Breast Oncology,The First People’s Hospital of Chenzhou City,Chenzhou 423000,China)

机构地区：[1]郴州市第一人民医院乳腺肿瘤内科,郴州423000

出　　处：《中国组织化学与细胞化学杂志》2024年第6期515-523,539,共10页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探讨MAGI2-反义RNA3(MAGI2-AS3)通过靶向微小RNA-934(miR-934)调控嗜乳脂蛋白亚家族3成员A3(BTN3A3)对神经内分泌肿瘤细胞增殖、迁移和侵袭能力的影响。方法通过转染过表达质粒、微小RNA模拟物(miR-mimic)或抑制剂(miR-inhibitor)等方法改变小鼠小肠神经内分泌肿瘤细胞(STC-1)内的MAGI2-AS3、miR-934或BTN3A3表达,qRT-PCR检测MAGI2-AS3、miR-934和BTN3A3m RNA的表达水平,Ed U检测细胞增殖,TUNEL检测细胞凋亡,Transwell和细胞划痕实验评估细胞侵袭和迁移能力,双荧光素酶实验验证miR-934与MAGI2-AS3及BTN3A3之间的靶向关系,FISH检测miR-934的表达量,免疫荧光检测BTN3A3的表达量。结果在STC-1细胞中,MAGI2-AS3和BTN3A3表达降低,miR-934表达升高。过表达MAGI2-AS3降低细胞的增殖率、侵袭、迁移及miR-934表达水平,增加细胞凋亡率及BTN3A3表达。MAGI2-AS3通过靶向miR-934调控BTN3A3的表达。上调miR-934表达增加细胞增殖率、侵袭和迁移,降低细胞凋亡率及BTN3A3表达。BTN3A3过表达逆转miR-934对细胞增殖、迁移及侵袭能力的促进作用,并增强细胞凋亡。结论MAGI2-AS3通过靶向miR-934调控BTN3A3基因的表达,抑制神经内分泌肿瘤细胞的增殖、迁移和侵袭,并促进其凋亡。Objective To explore the effect of MAGI2 antisense RNA 3(MAGI2-AS3)in regulating the expression of BTN3A3 by targeting microRNA-934(miR-934)on the proliferation,migration,and invasion of neuroendocrine tumor cells.Methods MAGI2-AS3,miR-934,or BTN3A3 expression in mouse small intestine neuroendocrine tumor cells(STC-1)was altered by transfection with overexpression plasmids,miR mimics,or inhibitors.The mRNA expression levels of MAGI2-AS3,miR-934,and BTN3A3 were detected by qRT-PCR.Cell proliferation was measured by EdU assay,apoptosis by TUNEL assay,and invasion and migration by Transwell and scratch assays.The target relationship between miR-934 and MAGI2-AS3 and BTN3A3 was verified by dual-luciferase assay.miR-934 expression was detected by FISH,and BTN3A3 expression was assessed by immunofluorescence.Results In STC-1 cells,MAGI2-AS3 and BTN3A3 expression was reduced,while miR-934 expression was elevated.Overexpression of MAGI2-AS3 decreased cell proliferation,invasion,migration,and miR-934 expression,while increasing cell apoptosis and BTN3A3 expression.MAGI2-AS3 regulates BTN3A3 expression by targeting miR-934.Upregulation of miR-934 expression increased cell proliferation,invasion,and migration,while decreasing cell apoptosis and BTN3A3 expression.BTN3A3 overexpression reversed the effects of miR-934 on cell proliferation,migration,and invasion,and enhanced apoptosis.Conclusion MAGI2-AS3 regulates BTN3A3 gene expression by targeting miR-934,thereby inhibiting the proliferation,migration,and invasion of neuroendocrine tumor cells,and promoting their apoptosis.

关 键 词：神经内分泌肿瘤 MAGI2-反义RNA3 微小RNA-934 嗜乳脂蛋白亚家族3成员A3 

分 类 号：R735[医药卫生—肿瘤]