基于表型性状及SSR标记的扁豆种质资源遗传多样性分析  

Genetic Diversity Analysis of Lablab purpureus Germplasm Resources Based on Morphological Trait and SSR Markers

作　　者：姚陆铭[1] 袁娟[2] 马晓红[1] 王彪[1] Yao Luming;Yuan Juan;Ma Xiaohong;Wang Biao(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Shanghai Pudong New District Agricultural Technology Extending Center,Shanghai 201201,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海市浦东新区农业技术推广中心,上海201201

出　　处：《作物杂志》2025年第1期35-45,共11页Crops

基　　金：上海市科技兴农项目(2021-02-08-00-12-F00766)。

摘　　要：利用17个表型性状及31对多态性SSR标记对收集自国内不同地区及印度、美国等国家的115份扁豆种质资源进行遗传多样性分析。结果表明,9个质量性状存在25种变异类型,多样性指数范围为0.23~1.19,平均多样性指数0.72;8个数量性状变异系数范围为8.97%~36.23%,大部分性状呈现出丰富的遗传多样性。主成分分析发现,9个主成分因子累计贡献率为77.39%,可反映扁豆资源的大部分遗传信息。31对SSR标记在115份扁豆材料中共检测到106个多态性位点,平均有效等位基因数目为1.96个,引物的多态性信息含量在0.05~0.69,群体多样性指数范围为0.05~0.74,平均为0.45。聚类分析发现,在欧式距离为3.8时可将扁豆资源分为6个类群,主成分分析发现不同类群相对独立,类群内个体分布则相对集中。群体遗传结构分析将扁豆资源分为2个类群。群体遗传分析方法均显示扁豆资源类群分类与其地理来源存在不一致性。结果表明扁豆种质资源具有较高的遗传多样性,研究结果为扁豆种质资源的利用及新品种的开发提够了重要参考。The genetic diversity of 115 Lablab purpureus germplasms from China,India and the United States was analyzed using 17 phenotypic traits and 31 pairs of polymorphic SSR markers.The results showed that a total of 25 types of variation were observed for the nine qualitative traits.The diversity index(H′)ranged from 0.23 to 1.19 with an average of 0.72.The coefficient of variation of eight quantitative traits was 8.97%-36.23%,and most of the phenotypic traits showed rich genetic diversity.Most of genetic information could represented by nine principle component factors with 77.39%of cumulative contribution rate according to principal component analysis(PCA).The 106 alleles were amplified by 31 pairs of SSR primers in L.purpureus germplasms with an average of 1.96 alleles per marker.The polymorphic information content(PIC)of each marker varied from 0.05 to 0.69,and the group genetic diversity indexes ranged from 0.05 to 0.74 with an average of 0.45.The unweighted pair-group method with arithmetic means(UPGMA)cluster analysis divided the L.purpureus germplasms into six groups at the genetic distance of 3.8.Additionally,according to PCA,materials in each group were distributed concentrated while each group was independent to each other.All tested materials were divided into two groups by population genetic structure analysis.There was no consistency between the cluster of L.purpureus germplasms and their geographical origin according to all the UPGMA,PCA and genetic structure analysis.This study demonstrated that L.purpureus germplasms had a high level of genetic diversity.These findings provided scientific basis for the utilization of germplasms resources and breeding of L.purpureus.

关 键 词：扁豆 种质资源 表型性状 SSR标记 遗传多样性 

分 类 号：S643.5[农业科学—蔬菜学]