高粱Dof基因家族鉴定及表达分析  

Identification and Expression Analysis of Dof Gene Family in Sorghum

作　　者：付森杰 宋迎辉[1] 朱灿灿[1] 叶珍言 秦娜[1] 代书桃[1] 刘海霞 王春义[1] 李君霞[1] Fu Senjie;Song Yinghui;Zhu Cancan;Ye Zhenyan;Qin Na;Dai Shutao;Liu Haixia;Wang Chunyi;Li Junxia(Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Changge Agriculture and Rural Bureau,Xuchang 461500,Henan,China)

机构地区：[1]河南省农业科学院粮食作物研究所,河南郑州450002 [2]长葛市农业农村局,河南许昌461500

出　　处：《作物杂志》2025年第1期54-65,共12页Crops

基　　金：中央引导地方科技发展资金(Z20221341070);河南省农业科学院自主创新项目(2023ZC014);农业农村部/财政部现代农业产业技术体系专项资金项目(nycytx-CARS-06);河南省良种联合攻关项目(2022010401);河南省农业科学院创新团队专项(2023TD036)。

摘　　要：DNA结合单锌指蛋白(DNA binding with one finger,Dof)是植物中特有的一类转录因子,参与调控植物组织分化、种子萌发、植物抗逆及代谢等生理生化过程。为鉴定并分析高粱Dof转录因子家族成员,利用生物信息学方法从全基因组水平对高粱Dof转录因子家族成员的基本基因结构、理化性质、保守基序、启动子顺式作用元件、组织特异性表达及干旱胁迫表达等进行分析。20%PEG6000模拟干旱胁迫处理豫粱10号的幼苗后,利用qPCR技术分析Dof基因家族成员响应不同时间段干旱胁迫的表达情况。结果共鉴定了30个高粱Dof基因,分布在9条染色体上,基因保守性分析结果显示,Motif1(即锌指结构)是高粱Dof基因家族中保守的蛋白质序列;其中29个基因中都含有CX2CX21CX2C保守基序,而SbDof15基因保守序列为CX20CX2C;启动子结构分析表明,Dof基因的启动子区域中含有ABA、MYB、MeJA、低温等逆境胁迫响应元件;进化树分析结果表明,高粱Dof蛋白与玉米的同源性更高,热图分析表明Dof家族成员在高粱的不同部位都有表达。qRT-PCR结果表明,高粱Dof基因家族成员可能参与高粱应对干旱胁迫的响应,不同的Dof基因成员在应对干旱胁迫时的调控机制可能不一样。DNA binding with one finger(Dof)is a unique transcription factor in plants,which is involved in regulating physiological and biochemical processes such as plant tissue differentiation,seed germination,stress resistance and metabolism.Using a bioinformatics approach,the basic gene structure,physical and chemical characteristics,conserved motifs,promoter cis-acting elements,tissue-specific expression,and drought-stress expression of the sorghum Dof transcription factor family members were examined at the whole genome level in order to identify and examine the members of the family.Following a 20%PEG6000 simulated drought stress treatment of Yuliang 10 seedlings,the expression of Dof gene family members in response to drought stress at various time points was examined using qPCR.Results showed that a total of 30 Dof genes were identified and distributed on nine chromosomes.The results of gene conservation analysis showed that Motif1(zinc finger structure)was the conserved protein sequence of sorghum Dof gene family.Among them,29 genes contained CX2CX21CX2C conserved motif,while the SbDof15 gene was CX20CX2C.The structure of promoter analysis showed that the promoter region of Dof contained ABA,MYB,MeJA,low temperature and other stress response elements.The results of evolutionary tree analysis showed that the homology of sorghum Dof protein was higher than that of maize,and heat map analysis showed that Dof family members were expressed in different parts of sorghum.The results of qRT-PCR indicated that Dof gene family members may be involved in the response of sorghum to drought stress,and different Dof gene members may have different regulatory mechanisms in response to drought stress.

关 键 词：高粱 Dof基因家族 转录因子 全基因组鉴定 PEG处理 

分 类 号：S514[农业科学—作物学]