绿豆种质资源萌发期耐盐性鉴定与评价  

Identification and Evaluation of Salt Tolerance of Mung Bean Germplasm Resources during Germination

作  者:时会影 范保杰[1] 刘长友[1] 王彦[1] 王珅 张志肖[1] 苏秋竹[1] 田静[1] Shi Huiying;Fan Baojie;Liu Changyou;Wang Yan;Wang Shen;Zhang Zhixiao;Su Qiuzhu;Tian Jing(Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Hebei Key Laboratory of Crop Genetics and Breeding,Shijiazhuang,050035,Hebei,China)

机构地区:[1]河北省农林科学院粮油作物研究所/河北省遗传育种重点实验室,河北石家庄050035

出  处:《作物杂志》2025年第1期66-75,共10页Crops

基  金:国家食用豆产业技术体系(CARS-08-G03);国家重点研发专项(2021YFD1600601);河北省杂粮杂豆种业创新团队(21326305D)。

摘  要:萌发期耐盐性是制约绿豆种质在盐碱地上应用的关键因素。以NaCl溶液模拟胁迫环境,对68个绿豆种质资源进行了耐盐性综合评价及筛选。结果表明,发芽势、发芽率、胚芽长等10项指标处理组与对照组间存在极显著差异(P<0.001),且10项指标的耐盐系数间均存在着不同程度的相关性(R2介于-0.120~0.947)。利用主成分分析将10项单项指标转化为3个综合指标,通过耐盐性综合评价和聚类分析把参试材料按耐盐性强弱分为3个类群,其中25份材料为高耐盐类型,20份材料为耐盐中间类型,23份材料为盐敏感类型。采用逐步回归建立绿豆萌发期耐盐性评价模型,筛选出子叶下部鲜重、活力指数、发芽势、发芽率、发芽指数、胚根长和胚芽鲜重7个指标,可用于不同绿豆种质资源耐盐性快速评价与预测。Salt tolerance during germination is an important factor restricting the application of mung bean germplasm in saline-alkali soil.The 68 salt tolerance mung bean germplasm resources was evaluated and screened with NaCl solution.The results showed that there were extremely significant differences in ten indexes between the treatment groups and the control group(P<0.001),such as germination potential,germination rate and germ length.The salt tolerance coefficients of the ten indexes were correlated to different degrees(R2 ranged from-0.120 to 0.947).Principal component analysis converted ten individual indexes into three comprehensive indexes,and the tested materials were divided into three categories according to the strength of salt tolerance through comprehensive evaluation of salt tolerance and cluster analysis,of which 25 materials were high salt tolerance types,20 materials were moderately salt tolerance types,and 23 materials were salt sensitive types.The salt tolerance evaluation model of mung bean at germination stage was established by step-to-step regression,and seven indexes including fresh weight,vigor index,germination potential,germination rate,radicle length and fresh weight of germ were selected,which can be used for rapid evaluation and prediction of salt tolerance of different mung bean germplasm resources.

关 键 词:绿豆 萌发期 耐盐性 综合评价 

分 类 号:S522[农业科学—作物学]

 

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