突变NtJAZ1基因获得高烟碱烤烟新材料  

Mutating NtJAZ1 Gene to Obtain High Nicotine Flue-Cured Tobacco New Material

作　　者：高玉龙[1] 赵璐 王丙武[1] 孔光辉[1] 王亚辉[1] 刘剑金 段杰 吴兴富[1] 李青 者开明 Gao Yulong;Zhao Lu;Wang Bingwu;Kong Guanghui;Wang Yahui;Liu Jianjin;Duan Jie;Wu Xingfu;Li Qing;Zhe Kaiming(Yunnan Academy of Tobacco Agricultural Sciences/National Center for Tobacco Gene Engineering,Kunming 650021,Yunnan,China;Puer Branch of Yunnan Provincial Tobacco Company,Puer 665000,Yunnan,China;Lincang Company Gengma Branch of Yunnan Provincial Tobacco Company,Lincang 677500,Yunnan,China)

机构地区：[1]云南省烟草农业科学研究院/国家烟草基因工程研究中心,云南昆明650021 [2]云南烟草公司普洱市公司,云南普洱665000 [3]云南省烟草公司临沧市公司耿马分公司,云南临沧677500

出　　处：《作物杂志》2025年第1期83-88,共6页Crops

基　　金：中国烟草总公司云南省公司科技计划项目(2021530000242029)。

摘　　要：烟碱是栽培烟草中重要的化学成分之一,深刻影响烟叶质量。为了培育高烟碱含量的烟草新材料,在我国主栽品种云烟87的甲基磺酸乙酯(EMS)突变体库中筛选烟碱合成负调控因子基因NtJAZ1的突变体材料。结果表明,在突变体库中筛选获得了12个突变体材料,其中10个突变导致NtJAZ1氨基酸变化,1份突变位点位于内含子,1个无义突变。温室种植纯合突变体材料,现蕾期检测整株叶片烟碱含量,其中2个株系(J8和J11)的烟碱含量显著高于对照。J11 I-1和J11Ⅳ-1打顶后其烟碱含量分别比对照提高48%和58%。qRT-PCR表明,NtPMT1a和NtQPT基因在突变体J11 I-1根中的表达水平显著升高。综上,筛选获得的高烟碱突变体材料J11不涉及转基因成分,可为培育高烟碱含量烤烟新品种提供种质资源。Nicotine is one of the most important chemical compounds in cultivated tobacco,which largely determines the quality of tobacco leaves.In order to develop a new tobacco material with high nicotine content,the mutant material of NtJAZ1,a negative regulator of nicotine synthesis,was screened from the ethyl methyl sulfonate(EMS)mutant library of Yunyan 87,a major cultivar in China.The results showed that 12 mutant materials were selected from the mutant library,of which ten mutations resulted in the amino acid change of NtJAZ1,one mutation was located in the intron,and one nonsense mutation.The homozygous mutant material was planted in greenhouse.The nicotine contents in leaves of two strains(J8 and J11)were significantly higher than that of the control at bud stage.After topping,the nicotine content of J11 I-1 and J11 IⅤ-1 increased by 48%and 58%,respectively,compared with the control.qRT-PCR showed that the expression levels of NtPMT1a and NtQPT genes in mutant J11 I-1 were significantly increased.In conclusion,the high-nicotine mutant material J11 obtained by screening does not involve transgenic components,which can provide germplasm resources for breeding new varieties of flue-cured tobacco with high nicotine content.

关 键 词：烤烟 烟碱 甲基磺酸乙酯(EMS) NtJAZ1 

分 类 号：S572[农业科学—烟草工业]