HPLC-ELSD法同时测定党参休眠过程中5种可溶性糖含量  

Simultaneous Determination of Contents of Five Kinds of Soluble Sugars in Codonopsis pilosula during Dormancy by HPLC-ELSD Method

作　　者：唐顺莉 张延红 杜弢 陈晖 何春雨 董婉琦 Tang Shunli;Zhang Yanhong;Du Tao;Chen Hui;He Chunyu;Dong Wanqi(Department of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000

出　　处：《作物杂志》2025年第1期202-207,共6页Crops

基　　金：国家自然科学基金(81960683);甘肃省高等学校产业支撑计划项目(2020C-09);甘肃省科技创新服务平台建设项(18JR2TA017);甘肃省民生科技专项(科技特派员专题)(20CX9NA070);中央引导地方科技发展专项资金项目(30440323);现代农业产业技术体系建设专项资金(CARS-21)。

摘　　要：建立高效液相色谱―蒸发光散射(HPLC-ELSD)法同时测定党参5种可溶性糖含量的技术方法,探明可溶性糖在党参抗寒及休眠过程中发挥的作用。5种可溶性糖适宜的检测条件为Analysis Column(4.6 mm×250 mm 5-Micron)色谱柱,流动相为乙腈:水(70:30)溶液,柱温35℃,流速1.0 m L/min;ELSD参数:雾化温度50℃,漂移管温度110℃,氮气流速2.2 m L/min,增益值1.0。在党参自然休眠过程中海藻糖只在休眠前期的根和休眠期的芽中检测到;棉子糖只在休眠前期的根中检测到;果糖和葡萄糖在休眠前期的叶中含量较高,蔗糖在休眠前期的根和休眠期的芽中含量较高,三者均在解除休眠后的芽中含量最高。本研究建立了高效的HPLC-ELSD法同时检测5种可溶性糖的技术体系,明确了海藻糖和棉子糖是党参抗寒的主要可溶性糖组分,其中海藻糖在根中合成并进一步转运至芽中。果糖、葡萄糖和蔗糖是党参根中储存的可溶性糖组分,也是萌发的物质和能量来源。A method was developed for the simultaneous determination of contents of five soluble sugars in different organs of Codonopsis pilosula(Franch.)Nannf.during natural dormancy by HPLC-ELSD for investigating the role of soluble sugars in the cold resistance.The suitable detection conditions for the five soluble sugars were:Analysis Column(4.6 mm×250 mm 5-Micron),acetonitrile:water(70:30)solution as the mobile phase,column temperature of 35°C,flow rate of 1.0 mL/min;ELSD parameters:nebulization temperature of 50°C,drift tube temperature of 110°C,nitrogen flow rate of 2.2 mL/min,gain value of 1.0.During the natural dormancy of C.pilosula,trehalose was only detected in roots(pre-dormant)and buds(dormant),raffinose was only detected in pre-dormant roots;fructose and glucose were found in higher levels in the leaves in the early stage of dormancy,and sucrose was found in higher levels in the roots in the early stage of dormancy and the buds in the dormant period.The highest levels of all three types of sugars were found in the buds after dormancy was released.This study established an efficient HPLC-ELSD technical system for the simultaneous detection of five soluble sugars,and confirmed that trehalose and raffinose are the main soluble sugar components of C.pilosula for cold resistance.Trehalose is synthesized in the roots and further transported to the buds.Fructose,glucose and sucrose are soluble sugar components stored in the roots of C.pilosula,and are also the source of substances and energy for germination.

关 键 词：党参 可溶性糖组分 高效液相色谱―蒸发光散射法 休眠 逆境生理 

分 类 号：S567.53[农业科学—中草药栽培]