液相色谱-串联质谱法测定豆制品中碱性橙2  

Determination of Basic Orange 2 in Soybean Products by LC-MS/MS

作　　者：田云飞 王银辉 李龙宣 刘俊 李影 郭文杰 TIAN Yunfei;WANG Yinhui;LI Longxuan;LIU Jun;LI Ying;GUO Wenjie(Anhui Runan Xinke Testing Technology Co.,Ltd.,Bozhou 236820,Anhui,China;Anhui GujingGongjiu Co.,Ltd.,Bozhou 236820,Anhui,China)

机构地区：[1]安徽润安信科检测科技有限公司,安徽亳州236820 [2]安徽古井贡酒股份有限公司,安徽亳州236820

出　　处：《酿酒》2025年第2期64-68,共5页Liquor Making

摘　　要：建立液相色谱-串联质谱法测定豆制品中碱性橙2的分析方法。样品经水-乙酸-乙酸铵-乙腈超声提取、离心、过滤膜后直接进样,采用Accucore a Q色谱柱(2.1×100 mm,2.6μm)分离,正离子模式扫描,外标法定量。结果表明碱性橙2在0.5~15.0 ng/m L范围内呈良好线性,相关系数(R2)≥0.99,其仪器检出限为为1.2μg/kg,定量限为4.0μg/kg;为了验证方法的精密度和准确度,做了重现性实验和加标回收实验,其RSD为1.91%~8.93%,回收率为80.6%~98.2%。本文适用于豆制品中碱性橙2的快速筛查和定量分析,方法前处理简单,测定快、准、灵敏度高。Establish an analytical method for the determination of basic orange 2 in soybean products using liquid chromatography tandem mass spectrometry.The sample was extracted by water acetic acid ammonium acetate acetonitrile ultrasound,centrifuged,and filtered before direct injection using an Accucore aQ chromatography column(2.1×100 mm,2.6μm)separation,positive ion mode scanning,quantitative analysis using external standard method.The results showed that basic orange 2 exhibited good linearity in the range of 0.5 to 15.0 ng/mL,with a correlation coefficient(R2)≥0.99,and an instrument detection limit of 1.2μg/kg,with a quantification limit of 4.0μg/kg;To verify the precision and accuracy of the method,reproducibility experiments and spiked recovery experiments were conducted,with RSD ranging from 1.91%to 8.93%and recovery rates ranging from 80.6%to 98.2%.This article is applicable for the rapid screening and quantitative analysis of basic orange 2 in soybean products.The method has simple pre-treatment,fast,accurate,and high sensitivity.

关 键 词：超高效液相色谱-串联质谱法 豆制品 工业染料 碱性橙2 检测 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] TS207.3[轻工技术与工程—食品科学与工程]