红芪多糖超声提取工艺优化及抗氧化、美白和吸湿保湿活性  

Optimization of ultrasonic extraction process for polysaccharides from Radix Hedysari and their antioxidant,whitening,moisture absorption and moisturizing activities

作　　者：原梦瑶 康淑荷 崔璐娟[1,2,3,4] 朱青青 李胜硕[1,2,3,4] 郭金旺 YUAN Mengyao;KANG Shuhe;CUI Lujuan;ZHU Qingqing;LI Shengshuo;GUO Jinwang(Key Laboratory of Environment-Friendly Composites of the State Ethnic Affairs Commission,Lanzhou 730106,Gansu,China;Gansu Provincial Biomass Function Composites Engineering Research Center,Lanzhou 730106,Gansu,China;Key Laboratory for Utility of Environment-Friendly Composite Materials and Biomass in University of Gansu Province,Lanzhou 730106,Gansu,China;School of Chemical Engineering,Northwest Minzu University,Lanzhou 730106,Gansu,China)

机构地区：[1]环境友好复合材料国家民委重点实验室,甘肃兰州730106 [2]甘肃省生物质功能复合材料工程研究中心,甘肃兰州730106 [3]甘肃省高校环境友好复合材料及生物质利用省级重点实验室,甘肃兰州730106 [4]西北民族大学化工学院,甘肃兰州730106

出　　处：《精细化工》2025年第3期577-586,共10页Fine Chemicals

基　　金：中央高校基本科研业务费专项资金项目(31920230144);国家自然科学基金项目(21968032、22165025)。

摘　　要：基于单因素〔超声时间、超声温度、料液比(g∶mL,下同)、提取次数、粒度〕超声辅助水提取法提取红芪多糖的实验结果,选取超声时间、超声温度、料液比采用响应面法优化了红芪多糖的提取工艺,考察了红芪多糖对2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(ABTS^(+)•)、1,1-二苯基-2-苦基肼自由基(DPPH•)、超氧阴离子自由基(•O_(2)^(–))和•OH的清除能力、对酪氨酸酶单酚酶的抑制作用及吸湿保湿性能。结果表明,超声辅助水对粒度45目的红芪粉末提取红芪多糖,在超声功率99 W、超声时间43 min、超声温度70℃、料液比1∶21、提取3次的最佳工艺条件下,多糖提取率为23.90%;质量浓度1.00 g/L红芪多糖对ABTS^(+)•清除率89.67%,半数抑制浓度(IC50)为0.40 g/L;质量浓度10.00 g/L红芪多糖对酪氨酸酶单酚酶的抑制率为54.14%,IC50为8.06 g/L;12 h内,在相对湿度43%的环境中,红芪多糖的吸湿率(红芪多糖吸水后的总质量占吸水前红芪多糖质量的百分数)为101.01%,质量浓度10 g/L红芪多糖溶液保湿率达99.87%。Based on the experimental results of single factors[ultrasonic time,ultrasonic temperature,solid-liquid ratio(g∶mL,the same below),extraction times,particle size]in ultrasonic assisted water extraction of polysaccharides from Radix Hedysari,response surface method was used to optimize the extraction process of Radix Hedysari polysaccharides using ultrasonic time,ultrasonic temperature and solid-liquid ratio as variables.The scavenging capabilities of Radix Hedysari polysaccharides on 2,2'-diazo-di(3-ethylbenzothiazole-6-sulfonic acid)diamine salts free radicals(ABTS^(+)•),1,1-diphenyl-2-trinitrophenylhydrazine radicals(DPPH•),superoxide anion free radicals(•O_(2)^(–))and•OH,as well as the inhibition on tyrosinase monophenolase and hygroscopic as well as moisturizing properties were investigated.The results showed that under the optimal conditions of ultrasonic power 99 W,ultrasonic time 43 min,ultrasonic temperature 70℃,solid-liquid ratio 1∶21,extraction times 3 times,the extraction yield of polysaccharides was 23.90%.The scavenging rate of ABTS^(+)•by Radix Hedysari polysaccharides with a mass concentration of 1.00 g/L was 89.67%,and the half inhibitory concentration(IC50)was 0.40 g/L.The inhibitory rate of Radix Hedysari polysaccharides with a mass concentration of 10.00 g/L on tyrosinase monophenolase was 54.14%,and the IC50 was 8.06 g/L.Within 12 h,in an environment with a relative humidity of 43%,the moisture absorption rate of Radix Hedysari polysaccharides(the percentage of the total mass of Radix Hedysari polysaccharides after water absorption to the mass of polysaccharides before water absorption)was 101.01%,and the moisture retention rate of Radix Hedysari polysaccharides reached 99.87%at a mass concentration of 10 g/L.

关 键 词：红芪 多糖 提取 响应面法 抗氧化 美白 吸湿保湿 中药现代化技术 

分 类 号：R284.2[医药卫生—中药学] TQ658[医药卫生—中医学]