甜瓜CmPIPs基因家族鉴定和表达模式分析  

Identification and Expression Pattern Analysis of CmPIPs Gene Family in Melon

作　　者：贺姜姜 金兰 金苏日古嘎 李永 红雨[1,2] 高峰 HE Jiangjiang;JIN Lan;JIN Suriguga;LI Yong;HONG Yu;GAO Feng(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022,China;Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region,Hohhot 010022,China;Key Laboratory of Infinite-Dimensional Hamiltonian System and Its Algorithm Application,Ministry of Education,Hohhot 010022,China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022 [2]内蒙古自治区高等学校蒙古高原生物多样性保护与可持续利用重点实验室,内蒙古呼和浩特010022 [3]无穷维哈密顿系统及其算法应用教育部重点实验室,内蒙古呼和浩特010022

出　　处：《华北农学报》2025年第1期53-62,共10页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31660577);内蒙古师范大学研究生科研项目(S20231079Z);内蒙古师范大学基本科研业务费专项资金项目(2022JBTD010);无穷维哈密顿系统及其算法应用教育部重点实验室开放课题项目(2023KFYB04)。

摘　　要：对甜瓜CmPIPs基因家族鉴定和表达模式分析,为进一步探究甜瓜CmPIPs基因家族功能及甜瓜遗传改良提供理论依据和支持。利用TBtools、MEME、MEGA X、Plant-CARE工具对CmPIPs进行生物信息学分析,利用GraphPad Prism 10软件对CmPIP2;7在甜瓜授粉后不同时期中果皮中的表达量以及CmPIPs各成员在不同组织和不同浓度的植物激素处理幼叶中的表达量进行可视化解析。结果显示,CmPIP2;7与CsPIP2;8亲缘关系最近;12个CmPIPs家族成员主要分布在1,3,4,5,9,10,11号染色体上;除CmPIP2;8有3个外显子区,其余成员均有4个外显子区;CmPIPs各成员的启动子区域有多个顺式作用元件,如生长素、赤霉素、脱落酸等激素应答元件;CmPIP2;7在甜瓜的快速生长期以及成熟期的表达量显著上调;CmPIPs各家族成员在甜瓜的不同组织中均有表达;生长素浓度为40.0μmol/L时,CmPIP2;4表达量显著上调,CmPIP1;1、CmPIP2;1、CmPIP2;2和CmPIP2;3的表达量极显著下调;脱落酸浓度为0.4,4.0,40.0μmol/L时,CmPIP1;1、CmPIP2;1、CmPIP2;3、CmPIP2;9表达量均显著下调;茉莉酸甲酯浓度为44.640μmol/L时,CmPIP2;1和CmPIP2;5表达量显著下调,CmPIP2;2、CmPIP2;3、CmPIP2;7、CmPIP2;9表达量显著上调;乙烯利浓度为4.0 mmol/L时,CmPIPs各成员表达量均显著上调。明确了CmPIPs基因家族成员的基因结构、序列特征、进化关系以及共线性关系,对其表达模式进行了解析。The identification and expression pattern analysis of CmPIPs gene family in melon could provide theoretical basis and support for further exploring the function of CmPIPs gene family and the genetic improvement of melon.TBtools,MEME,MEGA X and Plant-CARE tools were used to analyze the bioinformatics of CmPIPs,and the expression level of CmPIP2;7 in the pericarp of melon at different stages after pollination,and the expression level of each member of CmPIPs in different tissues and different concentrations of plant hormone treated young leaves were visualized in the software GraphPad Prism 10.The results showed that CmPIP2;7 and CsPIP2;8 had nearest kinship;the 12 members of the CmPIPs family were mainly distributed on chromosomes 1,3,4,5,9,10 and 11;except that CmPIP2;8 had 3 CDS regions,the other members had 4 CDS regions.The promoter regions of each member of CmPIPs had multiple cis-acting elements,hormone responsive elements,such as auxin,gibberellin,and abscisic acid.The expression level of CmPIP2;7 was significantly up-regulated during rapid development and maturity of melon fruit.Members of various families of CmPIPs were expressed in different tissues of melon.After treatment with 40.0μmol/L auxin,the expression level of CmPIP2;4 was significantly up-regulated,while the expression level of CmPIP1;1,CmPIP 2;1,CmPIP2;2 and CmPIP2;3 were extremely significantly down-regulated,and when the concentrations of abscisic acid were 0.4,4.0 and 40.0μmol/L,the expression level of CmPIP1;1,CmPIP2;1,CmPIP2;3,CmPIP2;9 were significantly down-regulated.After treatment with 44.640μmol/L methyl jasmonate,the expression level of CmPIP2;1 and CmPIP2;5 were significantly down-regulated,while the expression level of CmPIP2;2,CmPIP2;3,CmPIP2;7 and CmPIP2;9 were significantly up-regulated.When the concentration of ethylene glycol was 4.0 mmol/L,the expression level of each member of CmPIPs was significantly up-regulated.The gene structure,sequence characteristics,evolutionary relationship and collinearity of CmPIPs gene family

关 键 词：甜瓜 基因家族 质膜内在蛋白 植物激素 表达特性 

分 类 号：S652.03[农业科学—果树学] Q78[农业科学—园艺学]