绣球WRKY基因家族全基因组鉴定及其响应叶斑病的表达分析  

Genome-wide Characterization of WRKY Gene Family in Hydrangea macrophylla and Expression Analysis in Response to Leaf Spot Disease

作　　者：刘欣童 陈慧杰 陈双双 冯景 齐香玉 周惠民 金玉妍 孙明[2] 邓衍明[1,2] LIU Xintong;CHEN Huijie;CHEN Shuangshuang;FENG Jing;QI Xiangyu;ZHOU Huimin;JIN Yuyan;SUN Ming;DENG Yanming(Jiangsu Provincial Key Laboratory for the Genetics and Improvement of Horticultural Crops,Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;State Key Laboratory of Efficient Production of Forest Resources,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]江苏省农业科学院休闲农业研究所,江苏省高效园艺作物遗传改良重点实验室,江苏南京210014 [2]北京林业大学林木资源高效生产全国重点实验室,北京100083

出　　处：《华北农学报》2025年第1期93-103,共11页Acta Agriculturae Boreali-Sinica

基　　金：江苏省农业科技自主创新资金项目(CX(23)3062);江苏省种业振兴“揭榜挂帅”项目(JBGS(2021)097);中央财政林业科技推广示范项目(苏[2024]TG03)。

摘　　要：为了解析绣球WRKY转录因子家族的成员特征及其在绣球叶斑病应答中的作用,利用生物信息学方法,对绣球无尽夏基因组中的WRKY家族成员进行全基因组鉴定,并系统分析了蛋白质理化特征、基因结构、系统进化、共线性和家族成员在多主棒孢菌侵染下的表达模式。结果表明:绣球全基因组中共鉴定到84个非冗余的HmWRKY成员,均属于亲水性蛋白,在绣球18条染色体上呈现不均匀分布,编码氨基酸112~1 046个;所有成员可分为3个亚组,即GroupⅠ~GroupⅢ,均含有WRKYGQK和C2H2组成的DNA结合域;HmWRKY成员的序列长度变化较大,从512 bp到40 338 bp,并且检测到8个存在共线性的基因对,Ka/Ks的比值均小于1,说明HmWRKY家族在进化中趋于纯化选择;同时,18个HmWRKY成员在绣球叶斑病菌侵染后表现出显著差异表达的特性,其中9个上调表达、9个下调表达。以上结果表明,HmWRKY基因在绣球响应叶斑病中可能具有重要作用。To understand the member characteristics of the WRKY transcription factor family in hydrangea and their roles in response to leaf spot disease,this study utilized the bioinformatics method to characterize WRKY family members in Hydrangea macrophylla Endless Summer,and systematically analyzed the protein physicochemical features,gene structure,systematic evolution,collinearity and expression patterns under the infection with Corynespora cassiicola of WRKY family members.The results showed that there were 84 non-redundant HmWRKY members in H.macrophylla genome.All of the HmWRKYs were hydrophilic proteins and unevenly distributed on 18 chromosomes of H.macrophylla,encoding 112—1046 amino acids.In addition,HmWRKY members were divided into 3 subgroups(GroupⅠ—GroupⅢ),which contained a conserved DNA binding domain composed of WRKYGQK and C2H2.The sequence length of HmWRKY varies greatly from 512 bp to 40338 bp,and 8 collinear gene pairs with Ka/Ks ratios less than 1 were detected,indicating that the HmWRKY family experienced purification selection in evolution.18 HmWRKY members showed significantly differential expression after infection with C.cassiicola,of which 9 up-regulated and 9 down-regulated.The results indicated that these HmWRKY genes might play important roles in response to leaf spot disease of hydrangea.

关 键 词：绣球 WRKY基因家族 转录因子 叶斑病 多主棒孢菌 

分 类 号：S432[农业科学—植物病理学]