湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析  

Analysis of ANO5 Gene Polymorphism and Its Association with Fat Deposition Traits in Hu Sheep

作　　者：黄志强 王维民 张德印 赵源 张煜坤 徐丹 杨晓斌 马宗武 何丽娟 蔡有鑫 刘晓强 张小雪[1] HUANG Zhiqiang;WANG Weimin;ZHANG Deyin;ZHAO Yuan;ZHANG Yukun;XU Dan;YANG Xiaobin;MA Zongwu;HE Lijuan;CAI Youxin;LIU Xiaoqiang;ZHANG Xiaoxue(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]兰州大学草地农业科技学院,甘肃兰州730000

出　　处：《华北农学报》2025年第1期207-214,共8页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(32260818,32372850)。

摘　　要：茴香胺5(ANO5)是一种定位于肌膜和肌浆网的多通道膜蛋白,主要在肌膜修复和磷脂争夺中起作用,ANO5基因突变会导致颌骨发育不全以及各种肌肉疾病。为了探讨绵羊ANO5基因的单核苷酸多态性与脂肪沉积性状的关联性,选择健康且系谱清晰的1 005只湖羊公羔群体为研究对象,采用PCR扩增技术和KASPar分型技术对试验群体ANO5基因位点多态性进行检测,并与脂肪沉积性状进行关联分析,并利用qPCR分析ANO5基因在不同组织中的表达水平。结果表明,绵羊ANO5基因在湖羊多种组织中广泛表达,与其他组织相比,ANO5基因在心脏组织中的表达量最高。在绵羊ANO5基因第10内含子中检测到了g.58010 C>T多态位点的3种基因型CC、CT和TT。描述性统计结果表明,肾周脂肪质量与其他脂肪质量的差异最大,变异程度最高。相关分析结果表明,脂肪沉积相关性状与生长性状、饲料效率性状均呈正相关。关联分析结果表明,该多态位点与湖羊肾周脂肪质量及其相关性状呈显著关联。其中,CC基因型个体的肾周脂肪质量及其相对质量均显著低于TT基因型个体。综上所述,绵羊ANO5基因g.58010 C>T突变位点可作为湖羊肾周脂肪沉积性状的候选分子标记。Anoctamin 5(ANO5)is a multichannel membrane protein localized in the sarcoplasmic and sarcoplasmic reticulum that primarily plays a role in myosin membrane repair and phospholipid scrambling,mutations in the ANO5 gene can lead to jaw hypoplasia as well as various myopathies.It aimed to investigate the association of SNPs in the ANO5 gene with fat deposition traits in sheep.A population of 1005 healthy and clearly genealogical Hu sheep male lambs was selected for the study,and PCR amplification and KASPar typing techniques were used to detect the locus polymorphisms of the ANO5 gene in the experimental population and analyze the associations with fat deposition traits.The expression level of ANO5 gene in different tissues was analyzed by qPCR.The results showed that sheep ANO5 gene was widely expressed in a variety of tissues in Hu sheep,and the highest expression of ANO5 gene was found in heart tissue compared with other tissues.Three genotypes of CC,CT and TT with the g.58010 C>T polymorphic locus were detected in the 10th intron of the sheep ANO5 gene.Descriptive statistics showed that the perirenal fat weight was the most different and had the highest degree of variability compared with other fat weights.Correlation analysis showed that fat deposition related traits were positively correlated with growth and feed efficiency traits,and the results of association analyses showed that the polymorphic locus was significantly associated with perirenal fat weight and its related traits in the Hu sheep.Among them,the perirenal fat weight of individuals with CC genotype was significantly lower than that of individuals with TT genotype.In conclusion,the g.58010 C>T mutation locus of the ANO5 gene can be used as a candidate molecular marker for perirenal fat deposition traits in Hu sheep.

关 键 词：湖羊 茴香胺5 多态性 肾周脂肪 关联分析 

分 类 号：S823[农业科学—畜牧学]