流感疫苗血凝素含量测定方法的研究及应用进展  

Research and application progress of methods for determination of hemagglutinin content in influenza vaccine

作　　者：林勇健(综述) 吴业红(审校) LIN Yongjian;WU Yehong Vaccine(Research Department,Changchun Institute of Biological Products Co.,Ltd.,Changchun 130012,Jilin Province,China)

机构地区：[1]长春生物制品研究所有限责任公司疫苗研究室,吉林长春130012

出　　处：《中国生物制品学杂志》2025年第2期229-234,共6页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(20210204082YY)。

摘　　要：血凝素(haemagglutinin,HA)是流感病毒表面的一种糖蛋白,可在宿主体内诱导产生保护性免疫应答,是流感疫苗的主要抗原,其含量是反映流感疫苗效力的重要指标。流感疫苗HA含量常用的检测方法为单向免疫扩散法(single-radial immunodiffusion,SRID),但该方法需使用标准参考品,其制备时间较长,在大流行流感暴发初期,无法及时获得国际参考品,难以采用SRID法检测HA含量,严重阻碍疫苗的研发效率。因此,亟需研发具有良好准确性、灵敏性、重复性的快速检测方法,以替代SRID法,提高疫苗研发速度,应对流感疫情的暴发。本文就近年流感疫苗HA含量检测方法的研究及应用进展作一综述,以期为HA含量及其他抗原检测方法的研发提供思路。Hemagglutinin(HA)is a glycoprotein on the surface of influenza virus,which can induce protective immune responses in a variety of hosts.It is the main antigen of influenza vaccines,and its content is an important indicator of influenza vaccine efficacy.The common detection method for HA content in influenza vaccines is single-radial immunodiffusion(SRID),which requires standard reference reagents and needs long preparation time.However,in the early stage of the pandemic influenza outbreak,it was difficult to obtain international reference materials in time,and it was hard to use SRID method to detect HA content,which seriously hindered the efficiency of vaccine research and development.Therefore,there is an urgent need to develop rapid HA content assay methods with good accuracy,sensitivity,and reproducibility to replace SRID method,improve the speed of vaccine development,and cope with influenza epidemic outbreaks.In this paper,the research and application progress of HA content detection methods for influenza vaccines in recent years is summarized,with a view to providing ideas for the development of detection methods for HA content and other antigens.

关 键 词：流感疫苗 血凝素 单向免疫扩散法 高效液相色谱 表面等离子体共振成像 

分 类 号：R186[医药卫生—流行病学]