不同生产期皖西白鹅生殖激素及相关基因比较分析  

Comparative analysis of reproductive hormones and related genes in Wanxi white geese during different production periods

作　　者：刘彦彦 王佳琪 朱世纪 吴宇康 张宇豪 闻爱友[1] 刘文举[1] 龚争 王淑娟[1,2] LIU Yanyan;WANG Jiaqi;ZHU Shiji;WU Yukang;ZHANG Yuhao;WEN Aiyou;LIU Wenju;GONG Zheng;WANG Shujuan(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China;Anhui Province Key Laboratory of Animal Nutritional Regulation and Health,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100 [2]安徽省动物营养调控与健康重点实验室,安徽凤阳233100

出　　处：《安徽科技学院学报》2025年第2期22-29,共8页Journal of Anhui Science and Technology University

基　　金：安徽省重点研究与开发计划(202204c06020076);安徽省自然科学基金面上项目(2008085MC94);安徽省高校自然科学研究项目(KJ2021A0869);安徽省高校协同创新项目(GXXT-2023-105)。

摘　　要：探究不同生产期皖西白鹅血清激素水平和卵巢发育相关基因表达差异性。分别选取开产前、产蛋期和就巢期的皖西白鹅各4只作为研究对象,通过测定不同时期鹅血清激素水平,卵巢发育相关基因表达,对比分析各指标的差异及调控作用。结果表明,产蛋期皖西白鹅血清中E_(2)、MLT、P_(4)的水平高于开产前和就巢期(P<0.05),产蛋期皖西白鹅卵巢中MUSTN 1、FHL 1、FHL 2、GSC、P 2 RX 1、FN 1、RSPO 3、IGF 1基因表达水平高于开产前和就巢期(P<0.05);产蛋期CCNB 1、FOXM1基因表达水平低于开产前和就巢期(P<0.05),而开产前的表达水平显著高于就巢期。皖西白鹅产蛋期需要高浓度的E_(2)、MLT、P_(4)和高表达的发育相关的基因(MUSTN 1、FHL 1、FHL 2、GSC、P 2 RX 1、FN 1、RSPO 3和IGF 1)来调节卵巢的功能和卵泡发育,推测这些基因可能是影响鹅卵巢卵泡发育和产蛋的潜在重要基因。To investigate the difference in serum hormone levels and the expression of development-related genes to ovarian of Wanxi white geese during differentproduction periods.Four Wanxi white geese were selected before-laying,laying,nest stage,respectively.Through the determination of the levels of serum hormones in geese at different periods as well as the expression of genes associated with ovarian development,the differences and regulatory effects of various indicators were compared and analyzed.The results indicated that the serum levels of estradiol(E_(2)),melatonin(MLT),and progesterone(P_(4))were significantly higher during the laying compared to the before-laying and nest(P<0.05).Additionally,the expression levels of MUSTN 1,FHL1,FHL2,GSC,P2RX1,FN1,RSPO3,and IGF 1 genes in the ovary were significantly upregulated during the laying(P<0.05).In contrast,the expression of CCNB 1 and FOXM 1 genes was lower during the laying than in the before-laying and nest(P<0.05),with the highest levels observed in the before-laying.The results of the study indicated that high levels of E_(2),MLT,P_(4),and highly expressed development-related genes(MUSTN1,FHL1,FHL2,GSC,P2RX1,FN1,RSPO3,IGF 1)were required to regulate ovarian function and follicular development during laying in Wanxi white geese.These genes may serve as crucial regulators of ovarian follicle development and egg production,providing potential targets for future research on reproductive performance improvement in geese.

关 键 词：皖西白鹅 开产前 产蛋期 就巢期 激素 基因表达 

分 类 号：S835[农业科学—畜牧学]