高糖状态下miRNA hsa-let-7b-5p对HUVECs凋亡及Fas表达影响的研究  

Effects of miRNA hsa-let-7b-5p on FAS expressions and apoptosis of HUVECs in high glucose state

作　　者：房洁 晋群 韩淑芳 谈红 李彬 刘放 FANG Jie;JIN Qun;HAN Shufang;TAN Hong;LI Bin;LIU Fang(Department of Cardiology,The 960 Hospital of the Joint Logistics support Force of the Chinese people's Liberation Army,Jinan 250031,China)

机构地区：[1]解放军联勤保障部队第九六〇医院心血管内科,济南250031

出　　处：《空军航空医学》2025年第1期32-37,共6页AVIATION MEDICINE OF AIR FORCE

基　　金：山东省医药卫生科技重点项目(202403011093)。

摘　　要：目的探讨高糖状态下miRNA hsa-let-7b-5p对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)凋亡和凋亡相关因子(factor-related apoptosis,Fas)表达的影响。方法培养HUVECs细胞,分为对照组(培养基葡萄糖终浓度为5.5 mmol/L)、高糖组(培养基葡萄糖终浓度为33.0 mmol/L)、高糖+miRNA hsa-let-7b-5p mimic组(应用模拟物miRNA hsa-let-7b-5p mimic转染HUVECs,培养基葡萄糖终浓度为33.0 mmol/L);高糖+miRNA hsa-let-7b-5p inhibitor组(应用抑制物miRNA hsa-let-7b-5p inhibitor转染HUVECs,培养基葡萄糖终浓度为33.0 mmol/L)。分别用CCK-8法检测各组细胞存活率,蛋白免疫印迹法检测各组细胞Fas蛋白表达,RT-PCR检测各组细胞内miRNA hsa-let-7b-5p和Fas的mRNA表达,TUNEL染色检测各组细胞凋亡。结果高糖+miRNA hsa-let-7b-5pmimic组较高糖组HUVECs细胞活力增加(P<0.001)、凋亡减少(P<0.001)、Fas mRNA(P<0.001)和Fas蛋白(P<0.001)表达减少、hsa-let-7b-5p mRNA表达上调(P<0.001);高糖+miRNA hsa-let-7b-5p inhibitor组较高糖组HUVECs细胞活力减少(P<0.001)、凋亡增加(P<0.001)、FasmRNA(P<0.001)和Fas蛋白(P<0.001)表达增加、hsa-let-7b-5pmRNA表达下调(P=0.001)。结论miRNAhsa-let-7b-5p可抑制高糖状态下HUVECs凋亡,对高糖状态下的血管内皮起保护作用。Objective To investigate the effect of miRNA hsa-let-7b-5p on factor-related apoptosis(Fas)expressions and apoptosis of human umbilical vein endothelial cells(HUVECs)in high glucose state.Methods HUVECs were cultured and divided into the control group(at the medium glucose concentration of 5.5 mmol/L),high glucose group(at the medium glucose concentration of 33.0 mmol/L),high glucose+miRNA hsa-let-7b-5p mimic group(at the medium glucose concentration of 33.0 mmol/L+miRNA hsa-let-7b-5p mimic transfected cells),and high glucose+miRNA hsa-let-7b-5p inhibitor group(at the medium glucose concentration of 33.0 mmol/L+miRNA hsa-let-7b-5p inhibitor transfected cells).The proliferation viability of the four groups was detected by CCK8 assay,the protein level of Fas in each group of cells by Western blotting,and the mRNA levels of miRNA hsa-let-7b-5p and Fas in HUVECs by RT-PCR.TUNEL staining was performed to detect the apoptosis of cells in each group.Results The miRNA hsa-let-7b-5p mimic transfection increased the proliferative activity(P<0.001)but decreased the apoptotic rate(P<0.001)of the cells.The expression levels of Fas mRNA(P<0.001)and Fas protein(P<0.001)were decreased while the expression level of hsa-let-7b-5p mRNA was increased(P<0.001).The miRNA hsa-let-7b-5p inhibitor transfection decreased the proliferative activity(P<0.001)but increased the apoptotic rate(P<0.001)of the cells.The expression levels of Fas mRNA(P<0.001)and Fas protein(P<0.001)were increased while that of hsa-let-7b-5p mRNA was decreased(P=0.001).Conclusion In high glucose state,miRNA hsa-let-7b-5p may inhibit apopto sis of HUVECs and protect vascular endothelial cells against atherosclerosis.

关 键 词：动脉粥样硬化 糖尿病 人脐静脉内皮细胞 MICRORNAS 

分 类 号：R587[医药卫生—内分泌] R-33[医药卫生—内科学]