藿香⁃龙胆草药提取物改善HNEpC细胞炎症损伤和间质转化的机制  

Mechanism of agastache rugos⁃gentian herbal extract ameliorating inflammatory injury and mesenchymal transition in HNEpC cells

作　　者：孟欣 张晓敏[1] 韩雪[1] MENG Xin;ZHANG Xiaomin;HAN Xue(Department of Traditional Chinese Medicine,Children's Hospital Affiliated to Zhengzhou University,Zhengzhou,Henan,China,450000)

机构地区：[1]郑州大学附属儿童医院中医科,河南郑州450000

出　　处：《分子诊断与治疗杂志》2025年第1期141-144,149,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南省自然科学基金青年基金(212300410268)。

摘　　要：目的比较藿香⁃龙胆草药提取物对circ⁃0070934、miR⁃199a⁃5p和N⁃乙酰氨基葡萄糖转移酶(MGAT3)的影响,探究其对人鼻黏膜上皮细胞(HNEpC)炎症损伤和间质转化(EMT)的改善作用。方法培养HNEpC细胞株分为空白组,对照组和观察组,对照组和观察组添加一定浓度的脂多糖(LPS)构建慢性鼻窦炎(CRS)模型,观察组加入龙胆⁃藿香草药提取物进行干预培养。干预培养后,检测并比较各组的circ⁃0070934、miR⁃199a⁃5p、MGAT3、炎症因子、EMT标志物及细胞侵袭/迁移能力。结果干预培养后,circ⁃007093、MGAT的mRNA表达量为空白组>观察组>对照组,miR⁃199a⁃5p的mRNA表达量为空白组<观察组<对照组(P<0.05);干预培养后,MGAT、钙黏蛋白(E⁃cadherin)水平为空白组>观察组>对照组,白介素⁃4(Interleukin⁃4,IL⁃4)、白介素⁃13(Interleukin⁃13,IL⁃13)、α⁃平滑肌肌动蛋白(α⁃SMA)水平为空白组<观察组<对照组;与对照组相比,观察组HNEpC的侵袭和迁移明显减弱(P<0.05)。结论龙胆⁃藿香草药提取物可通过上调circ⁃0070934和MGAT3并下调miR⁃199a⁃5p的mRNA表达缓解HNEpC炎症损伤和EMT。Objective To compare the effects of aggosa rugosa gentiana herbal extract on circ⁃0070934,miR⁃199a⁃5p and N⁃acetylglucosamine transferase 3(MGAT3),and to explore its improvement effect on inflammatory injury and mesenchymal transition(EMT)of human nasal epithelial cells(HNEpC).Methods HNEpC cell lines were divided into three groups:blank group,control group and observation group.The control group and observation group were treated with a certain concentration of lipopolysaccharide(LPS)to establish a chronic sinusitis(CRS)model,while the observation group was additionally treated with gentianagastache herbal extract for intervention culture.After the intervention and culture,circ⁃0070934,miR⁃199a⁃5p,MGAT3,inflammatory factors,EMT markers,and cell invasion/migration ability were detected and compared in each group.Results After intervention and culture,the mRNA expression levels of circ⁃007093 and MGAT were blank group>observation group>control group,and the mRNA expression level of miR⁃199a⁃5p was blank group<observation group<control group(P<0.05).Following intervention,the levels of MGAT and E⁃cadherin were blank group>observation group>control group.The levels of interleukin⁃4(IL⁃4),interleukin⁃13(IL⁃13)andα⁃smooth muscle actin(α⁃SMA)were blank group<observation group<control group(P<0.05).Compared with the control group,the invasion and migration of HNEpC in the observation group were significantly weakened(P<0.05).Conclusion The gentian⁃agastache herbal extract can improve the inflammatory injury and mesenchymal transition of HNEpC by up⁃regulating circ⁃0070934 and MGAT3,while downregulating miR⁃199a⁃5p mRNA expression.

关 键 词：人鼻黏膜上皮细胞 草药提取物 炎症损伤 间质转化 慢性鼻窦炎 

分 类 号：R285[医药卫生—中药学]