丹参提取液的制备工艺优化及美白抗氧化活性研究  

Preparation of Salvia Miltiorrhiza Extract and Study on Its Whitening and Antioxidant Effects

作　　者：王媛 吴永玲 孙佳瑶 岳梦芯 黄莹 WANG Yuan;WU Yongling;SUN Jiayao;YUE Mengxin;HUANG Ying(College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo 726000,China;Shaanxi Qinling Industrial Technology Research Institute of Special Biological Resources,Shangluo 726000,China)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000 [2]陕西秦岭特色生物资源产业技术研究院,陕西商洛726000

出　　处：《山东化工》2025年第3期31-35,共5页Shandong Chemical Industry

基　　金：陕西省大学生创新创业训练计划项目(S202311396037);陕西基础科学(化学、生物学)研究院基础科学研究计划项目(批准号:23JHQ002)。

摘　　要：以丹参作为研究对象,通过单因素结合响应面试验优化其黄酮类化合物的提取方法,并明确其美白与抗氧化效果。试验结果表明丹参中黄酮最佳的提取条件为液固比8∶1(mL∶g)、乙醇体积分数70%、超声温度70℃,在最优提取工艺条件下,总黄酮的得率为18.52%。丹参提取液对DPPH自由基和ABTS自由基均表现出明显的清除能力,同时对酪氨酸酶有明显抑制作用。研究结果为丹参黄酮类物质的提取及其在美白抗氧化产品中的应用提供了科学依据。In this study,Salvia miltiorrhiza was selected as the research object to optimize the extraction method of flavonoids and evaluate its antioxidant and whitening effects.The specific effects of liquid-solid ratio,ethanol concentration,ultrasonic time and temperature were evaluated by single factor test and response surface analysis.The experimental data revealed that under the conditions of liquid-solid ratio 8∶1(mL∶g),ethanol concentration of 70% and ultrasonic temperature of 70℃,the extraction rate of flavonoids was the highest,reaching 18.52%.The crude polysaccharides in Salvia miltiorrhiza also showed effective scavenging of DPPH and ABTS free radicals,and showed significant inhibitory effect on tyrosinase activity.Although its free radical scavenging ability was slightly insufficient compared with the same concentration of vitamin C,the optimal extraction process was simple and effective,which provided a scientific basis for the further industrial utilization of salvia miltiorrhiza extract and its application in whitening and antioxidant products.

关 键 词：丹参提取物 响应面分析法 工艺优化 美白 抗氧化 

分 类 号：TQ461[化学工程—制药化工]