家畜日本血吸虫抗体检测试纸条的研制与应用  

Preparation and Application of a Colloidal Gold Strip for Detecting Antibodies Against Schistosoma japonicum in Domestic Animals

作　　者：甄岳 张志 李彦 孙学强 杨胜男 段笑笑 孙荣钊 邴啟政 Zhen Yue;Zhang Zhi;Li Yan;Sun Xueqiang;Yang Shengnan;Duan Xiaoxiao;Sun Rongzhao;Bing Qizheng(Qingdao Lijian Bio-tech Co.,Ltd.,Qingdao 266041,Shandong,China;Qingdao Center for Animal Disease Prevention and Control,Qingdao 266000,Shandong,China)

机构地区：[1]青岛立见生物科技有限公司,山东青岛266041 [2]青岛市动物疫病预防控制中心,山东青岛266000

出　　处：《中国动物检疫》2025年第2期95-99,共5页China Animal Health Inspection

基　　金：青岛市科技惠民示范专项(24-1-8-xdny-4-nsh)。

摘　　要：为建立一种检测家畜日本血吸虫抗体的快速精准方法,将胶体金标记的重组链球菌G蛋白(r-SPG)喷涂到玻璃纤维素膜作为金标垫,将日本血吸虫虫卵可溶性抗原(SEA)和小鼠抗His标签单克隆抗体(His-tag单抗)分别包被到硝酸纤维素膜上作为检测线和质控线,制备了胶体金试纸条,然后开展了敏感性、特异性、重复性、符合率和临床应用研究。结果显示:该试纸条的诊断敏感性为96.43%,与土耳其斯坦分体吸虫、羊捻转血矛线虫、大片吸虫的阳性血清无交叉反应,批内、批间重复性良好,可在5~10 min完成检测。用1500份牛羊血清进行临床检测应用,发现试纸条与粪便毛蚴孵化法的总符合率达98.1%。结果表明,本研究制备的抗体检测试纸条敏感性高,特异性及重复性好,可为我国家畜日本血吸虫病防治工作提供有力的技术支持。In order to rapidly and accurately detect antibodies against Schistosoma japonicum in livestock,a kind of colloidal gold strip was prepared through spraying recombinant streptococcal G protein(r-SPG)labeled by colloidal gold onto a glass cellulose membrane as a gold-labeled pad,and encapsulating soluble egg antigens(SEA)and antiHis tag mouse monoclonal antibodies(His-tag mAb)onto nitrocellulose membrane as a detection line and a quality control line,respectively,then the sensitivity,specificity,reproducibility,compliance rate and clinical application were evaluated.The results showed that the strip was with 96.43%sensitivity of diagnosis,failed to crossly react with the positive sera of Ornithobilharzia turkistanicum,Haemonchus contortus and Fasciola gigantica,and with good intra-and inter-batch repeatability.It could complete detection in 5-10 minutes.1500 bovine and ovine sera were clinically tested,the total compliance rate of the strip with Schistosoma japonicum miracidium hatching method was found to be up to 98.1%.In conclusion,the strip was with high sensitivity,good specificity and reproducibility,and could strongly support the prevention and control of livestock Schistosomiasis japonicum in China.

关 键 词：日本血吸虫 胶体金 试纸条 抗体检测 

分 类 号：S851[农业科学—预防兽医学]