云南省西双版纳地区茶花鸡后口吸虫的鉴定与遗传进化分析  

Identification and Phylogenetic Analysis of Postharmostomum gallinum in Xishuangbanna Autonomous Prefecture,Yunnan Province

作　　者：唐玲 朱劼垚 侯明鹏 张绍云 李书宁 闫晓霞 魏炜 李布宾 刘孝刚 Tang Ling;Zhu Jieyao;Hou Mingpeng;Zhang Shaoyun;Li Shuning;Yan Xiaoxia;Wei Wei;Li Bubin;Liu Xiaogang(Xishuangbanna Vocational and Technical College,Jinghong 666100,Yunnan,China;Xishuangbanna Center for Animal Disease Prevention and Control,Jinghong 666100,Yunnan,China;College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Jinzhou 121000,Liaoning,China)

机构地区：[1]西双版纳职业技术学院,云南景洪666100 [2]西双版纳州动物疫病预防控制中心,云南景洪666100 [3]锦州医科大学畜牧兽医学院,辽宁锦州121000

出　　处：《中国动物检疫》2025年第2期120-124,共5页China Animal Health Inspection

基　　金：云南省教育厅科学研究基金项目(2024J1682)。

摘　　要：为确定云南省西双版纳地区茶花鸡盲肠分离到的吸虫种类及遗传进化关系,对高校实践课程中发现的茶花鸡盲肠吸虫进行形态学鉴定、PCR扩增测序、序列同源性分析及遗传进化分析。结果显示:吸虫虫体和虫卵形态结构均符合鸡后口吸虫特征;虫体ITS2序列与鸡后口吸虫(MH915391.1)相似性为99.26%,虫体COX1序列与鸡后口吸虫(NC_044643.1)相似性为98.99%;分离的吸虫与鸡后口吸虫聚于同一个分支。结果表明,西双版纳地区传入了鸡后口吸虫。提示未来应加强本地区禽群中的鸡后口吸虫监测和定期驱虫。In order to determine the species and phylogenetic relationship of trematodes isolated from caeca of camellia chicken in Xishuangbanna Autonomous Prefecture,Yunnan Province,morphological identification,PCR amplification and sequencing,sequence homology and phylogenetic analysis were conducted for trematodes that were found in practice courses of higher education institutions.The results showed that both the body and egg morphological structure of trematodes were consistent with the characteristics of Postharmostomum gallinum;the ITS2 sequence was 99.26%similarity to Postharmostomum gallinum(MH915391.1),while COX1 sequence was 98.99%to Postharmostomum gallinum(NC_044643.1);and the isolated trematodes were clustered in the same branch as Postharmostomum gallinum.In conclusion,Postharmostomum gallinum had been introduced to Xishuangbanna,which required to strengthen monitoring and regular deworming in poultry flocks in the region.

关 键 词：西双版纳茶花鸡 鸡后口吸虫 分子鉴定 遗传进化分析 

分 类 号：S851.3[农业科学—预防兽医学]