基于适配体封盖介孔材料的电化学传感器检测猪肉中磺胺二甲嘧啶  

Electrochemical biosensor based on aptamer-capped mesoporous material for sulfamethazine in porks

作　　者：郭婷[1,2] 黄馨瑞 周莹 张宇昊 刘晓竹[4] 马良 GUO Ting;HUANG Xinrui;ZHOU Ying;ZHANG Yuhao;LIU Xiaozhu;MA Liang(College of Food Science,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Specialty Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China;Key Laboratory of Condiment Supervision Technology for State Market Regulation,Chongqing 401121,China;Foshan Micro Miracles Biotechnology Company,Foshan 528000,China)

机构地区：[1]西南大学食品科学学院,重庆400715 [2]川渝共建特色食品重庆市重点实验室,重庆400715 [3]国家市场监管重点实验室(调味品监管技术),重庆401121 [4]佛山微奥云生物技术有限公司,佛山528000

出　　处：《食品与发酵工业》2025年第6期294-299,共6页Food and Fermentation Industries

基　　金：重庆市技术创新与应用发展专项重点项目(CSTB2023TIAD-KPX0066,CSTB2024TIAD-KPX0106);中央高校基本科研业务费专项资金资助项目(SWU-XJPY202310)。

摘　　要：磺胺二甲嘧啶(sulfamethazine,SMZ)作为磺胺类兽药的重要代表,因其广谱、杀菌力强等特点,广泛用于动物源性食品,已成为我国市场监管的重点之一。因此,该研究采用SMZ特异性适配体为目标识别探针,亚甲基蓝(methylene blue,MB)为信号增强探针,介孔二氧化硅纳米球(mesoporous silica nanospheres,MSN)为电化学信号增强探针载体,构建基于适配体封盖介孔材料的电化学传感器检测猪肉中SMZ。加入SMZ后,适配体特异性识别SMZ,引起适配体构象变化实现介孔材料中MB的快速释放,MB促进电子传递,造成电流强度增加,根据电流强度与目标物含量的相关关系对SMZ进行定量测定。结果表明,增量电流与SMZ浓度的对数在5 pg/mL~10 ng/mL有较好的线性关系,该方法灵敏度高,检测限为1.024 pg/mL,在实际猪肉样品中的加标回收率为98.3%~100.2%。该电化学传感器具有快速、灵敏、便捷等优点,为市场监管过程中现场快速检测筛查技术开发提供了一定的支撑和基础。Sulfamethazine(SMZ)as an important representative of sulfonamides is widely used in animal-derived food due to its advantages of broad spectrum and strong sterilizing ability,and it has become one of the keys of market supervision in China.Therefore,in this study,the electrochemical biosensor was constructed based on aptamers capping the mesoporous material for the detection of SMZ.In the biosensor,SMZ-specific aptamer was chosen as the target recognition probe,methylene blue(MB)as the signal enhancement probe,and mesoporous silica nanospheres(MSN)as the carrier of the electrochemical signal enhancement probe.In the presence of the SMZ,the release of MB in MSN was achieved through the conformational change due to the recognition of the aptamer and SMZ.MB promoted the electron transfer,resulting in the increase of current intensity.The quantitative determination of SMZ was conducted based on the correlation between current intensity and target concentration.Results showed that a good linear relationship between the incremental current and the logarithm of SMZ concentration had been exhibited in the range of 5 pg/mL to 10 ng/mL.This method had a high sensitivity with a detection limit of 1.024 pg/mL,and the percentage of spiked recoveries in pork samples was 98.3%-100.2%.The electrochemical biosensor had advantages such as rapidity,sensitivity,and convenience,providing essential support and foundation for the development of on-site rapid detection and screening technology in the market supervision process.

关 键 词：磺胺二甲嘧啶 适配体 介孔二氧化硅纳米材料 电化学 无标记 

分 类 号：TS251.51[轻工技术与工程—农产品加工及贮藏工程] O657.1[轻工技术与工程—食品科学与工程]