基于网络药理学及实验验证探讨健艾康浓缩丸对艾滋病的作用机制  

Mechanism of Jianaikang condensed pill capsules in treatment of AIDS on network pharmacology and experimental verification

作　　者：兰燕玲 苏琛[2] 杨琴 王玉璐 苗丁山 张涛[2] LAN Yanling;SU Chen;YANG Qin(Dazhou Vocational and Technical College,Sichuan,Dazhou 635000,China;Sichuan Academy of Chinese Medical Sciences,Sichuan,Chengdu 610041,China;Chengdu University of Traditional Chinese Medicine,Sichuan,Chengdu 611137,China)

机构地区：[1]达州职业技术学院,四川达州635000 [2]四川省中医药科学院,四川成都610041 [3]成都中医学大学,四川成都611137

出　　处：《四川中医》2025年第2期92-97,共6页Journal of Sichuan of Traditional Chinese Medicine

基　　金：四川省中医药管理局科学技术研究专项课题(编号:2023MS224)。

摘　　要：目的:基于网络药理学及临床实验研究健艾康浓缩丸治疗艾滋病的作用机制。方法:通过TCMSP、ETCM、PubChem、Swiss Target Prediction数据库筛选出健艾康浓缩丸的主要活性成分及靶点,在GeneCards、OMIM、TTD、DrugBank数据库获取疾病靶点,利用Venny 2.1.0在线工具绘制药物与疾病靶点的韦恩图,获得交集靶点,借助STRING数据库进行蛋白质相互作用分析;利用Cytpscape 3.10.1软件构建药物-活性成分-交集靶点网络图、蛋白质相互作用网络;在David数据库对核心效应靶点进行GO与KEGG功能的富集分析。检测艾滋病健艾康组和对照组患者治疗前后的血清IL-17、TNF-α和IFN-γ计数。结果:经过初步筛选,获得槲皮素、山奈酚、戈米辛B、塞拉苯嗪等90个活性成分和509个潜在靶点,其中与艾滋病的交集靶点有172个,核心靶点包括TP53、TNF、AKT1、ACTB、IL-6、CASP3、IL-1B、BCL-2、EGFR、NF-κB1等;GO富集在BP中主要包正向调控、细胞凋亡过程的负调控、促进蛋白质磷酸化等;MF主要包括ATP结合、酶结合、蛋白质结合等;CC主要包括核蛋白质复合物、染色质、细胞质等;KEGG富集发现IL-17、PI3K/AKT、TNF等信号通路。临床试验中,与治疗前相比,健艾康组在治疗后IL-17、TNF-α和IFN-γ计数均明显升高(P<0.05),对照组指标有一定波动,但无明显差异(P>0.05);两组患者在治疗前后指标计数均无差异(P>0.05)。结论:健艾康浓缩丸在一定程度上能改善细胞因子IL-17、IFN-γ水平,改善免疫功能,其中发挥主要作用的机制可能是对IL-17通路及PI3K/Akt通路的调控。Objective:To study the mechanism of action of Jianaikang condensed pill in the treatment of AIDS based on network pharmacology and clinical trials.Methods:The main active ingredients and targets of Jianaikang condensed pill were screened by TCMSP,ETCM,PubChem and Swiss Target Prediction databases,and the disease targets were obtained from GeneCards,OMIM,TTD and DrugBank databases,and the Venny 2.1.0 online tool was used to draw the Wayne diagrams of the drug and the disease targets.Using Venny 2.1.0 online tool to draw the Wayne diagram of drug and disease target,obtain the intersection target,and carry out protein interaction analysis with the help of STRING database;using Cytpscape 3.10.1 software to construct the network diagram of drug-active ingredient-intersection target and protein interaction network;and carrying out GO and KEGG functional enrichment analysis of core effector targets in David database.Serum IL-17,TNF-αand IFN-γcounts were detected before and after treatment in patients in the AIDS Jianaikan group and the control group.Results:After preliminary screening,90active ingredients and 509potential targets such as quercetin,kaempferol,Gomisin B,and Celabenzine were obtained,among which 172targets intersected with AIDS,and the core targets included TP53,TNF,AKT1,ACTB,IL-6,CASP3,IL-1B,BCL-2,EGFR,and NF-κB1,etc;GO was enriched in BP positive regulation of gene expression,negative regulation of apoptotic process,promotion of inflammatory response,etc;MF mainly includes ATP-binding,enzyme-binding,protein-binding,etc.CC mainly includes extracellular space,chromatin,cytosolm,etc;KEGG-enriched discovery of signalling pathways,such as IL-17,PI3K/AKT,and TNF.In the clinical trial,compared with the pre-treatment,the counts of IL-17,TNF-αand IFN-γin the Jian Aikang group were significantly higher after treatment(P<0.05),while the indicators in the control group fluctuated to some extent,but there was no significant difference(P>0.05);the counts of the indicators in the two groups before and after the tr

关 键 词：艾滋病 健艾康浓缩丸 IL-17 PI3K/AKT 

分 类 号：R259[医药卫生—中西医结合]