痛风金黄凝胶贴膏对急性痛风性关节炎大鼠膝关节肿胀度、血清尿酸及炎症因子水平影响的实验研究  

Experimental study on the effects of Tongfeng Jinhuang gel plaster on knee swelling,uric acid levels,and inflammatory factors in rats with acute gouty arthritis

作　　者：杨亚 赵松梅 卢勇 章斌[2] YANG Ya;ZHAO Songmei;LU Yong(Ya’an Hospital of Traditional Chinese Medicine,Sichuan,Ya’an 625000,China)

机构地区：[1]雅安市中医医院,四川雅安625000 [2]雅安职业技术学院,四川雅安625000

出　　处：《四川中医》2025年第2期112-117,共6页Journal of Sichuan of Traditional Chinese Medicine

基　　金：四川省科技厅科技计划项目/雅安市2021年度省级转移支付研究专项(编号:22ZYZF0003)。

摘　　要：目的:观察痛风金黄凝胶贴膏对急性痛风性关节炎(acute gouty arthritis,AGA)大鼠模型的改善作用。方法:SD大鼠60只随机分为空白组(blank)、模型组(model)、秋水仙碱组(Colchicine,0.3mg·kg^(-1))、秋水仙碱+低剂量凝胶贴膏组(TFJH-L,0.3mg·kg^(-1)+72mg·kg^(-1))、秋水仙碱+中剂量凝胶贴膏组(TFJH-M,0.3mg·kg^(-1)+144mg·kg^(-1))、秋水仙碱+高剂量凝胶贴膏组(TFJH-H,0.3mg·kg^(-1)+288mg·kg^(-1))共6组,每组10只,并分别给予相应药物,blank组和model组给予等体积蒸馏水灌胃,连续7天。于给药第5天向各组(除blank组外)大鼠右膝关节腔内注射尿酸钠溶液(monosodium urate,MSU)(25g·L^(-1))0.2mL,制备AGA模型。Blank组关节腔内注射等体积无菌生理盐水。造模后继续给药2天,并在第7天取出膝关节滑膜组织,观察大鼠膝关节肿胀情况,测定血清中血清尿酸(serum uric acid,SUA)及C反应蛋白(C-reactive protein,CRP)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL^(-1)β)、白细胞介素-18(interleukin-18,IL^(-1)8)和白细胞介素-6(interleukin-6,IL-6)水平,观察膝关节病理形态变化。结果:与blank组比较,model组大鼠膝关节肿胀明显,血清中SUA、CRP、TNF-α、IL^(-1)β、IL^(-1)8和IL-6水平均明显升高(P<0.01),膝关节滑膜组织可见纤维组织增生明显、结构紊乱,大量炎性细胞浸润,血管充血。与model组比较,Colchicine组、TFJH-L组、TFJH-M组、TFJH-H组的关节肿胀程度明显减轻,血清SUA、CRP、TNF-α、IL^(-1)β、IL^(-1)8和IL-6水平均显著降低(P<0.05),Colchicine组膝关节滑膜组织滑膜增生及炎症浸润程度降低,血管充血缓解;TFJH-L组、TFJH-M组、TFJH-H组膝关节滑膜组织滑膜增生及炎症浸润程度明显降低,血管充血明显缓解。结论:痛风金黄凝胶贴膏能够有效缓解膝关节肿胀,降低炎症因子水平,改善膝关节滑膜增生、炎性细胞浸润,为急性痛风性关节炎的临床治疗提�Objective:This study aimed to assess the therapeutic effects of Tongfeng Jinhuang gel plaster on acute gouty arthritis(AGA)in a rat model.Methods:Sixty Sprague-Dawley rats were randomly assigned to six groups:blank group,model group,colchicine group(0.3mg·kg^(-1)),colchicine+low-dose gel plaster group(TFJH-L,0.3mg·kg^(-1)+72mg·kg^(-1)),colchicine+medium dose gel plaster group(TFJH-M,0.3mg·kg^(-1)+144mg·kg^(-1)),colchicine+high-dose gel plaster group(TFJH-H,0.3mg·kg^(-1)+288mg·kg^(-1)),with 10rats in each group.The blank group and model group were administered an equivalent volume of distilled water via gavage for a period of seven consecutive days.On the fifth day of the experiment,rats in all groups(excluding the control group)were injected with 0.2ml of monosodium urate(MSU)solution(25g·L^(-1))into the right knee joint cavity to induce acute gouty arthritis.The control group received an equivalent volume of sterile physiological saline injection.Following the induction of the model,medication was administered for an additional two days,and on the seventh day,synovial tissue from the knee joint was extracted to assess the extent of swelling in the rat knee joint.The serum levels of uric acid(SUA),C-reactive protein(CRP),tumor necrosis factorα(TNFα),interleukin-1β(IL^(-1)β),interleukin-18(IL^(-1)8),and interleukin-6(IL-6)were quantified,and the knee joint pathology was assessed.Results:The model group of rats exhibited significant knee joint swelling and elevated levels of SUA,CRP,TNF-α,IL^(-1)β,IL^(-1)8,and IL-6compared to the control group(P<0.01).Fibrous tissue proliferation and structural abnormalities were noted in the synovial tissue of the knee joint,accompanied by a substantial presence of inflammatory cells and blood vessel congestion.In comparison to the control group,the Colchicine group,TFJH-L group,TFJH-M group,and TFJH-H group exhibited significantly decreased joint swelling,as well as reduced levels of serum SUA,CRP,TNF-α,IL^(-1)β,IL^(-1)8,and IL-6(P<0.05).The administration of Colc

关 键 词：痛风金黄凝胶贴膏 急性痛风性关节炎 炎症因子 滑膜组织 关节肿胀 

分 类 号：R274[医药卫生—中医骨伤科学]