野黄芩苷调节PI3K/Akt/NF-κB信号通路对溃疡性结肠炎小鼠结肠炎症损伤的影响  

Effects of Scutellarin on Colonic Inflammatory Injury in Mice with Ulcerative Colitis by Regulating PI3K/Akt/NF-κB Signaling Pathway

作　　者：张卓 胡畔 ZHANG Zhuo;HU Pan(Dept.of Emergency,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430001 Hubei,China)

机构地区：[1]湖北省中医院急诊科,湖北武汉430001

出　　处：《广州中医药大学学报》2025年第3期718-725,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：武汉市中医药科研项目(编号:WZ22Z15)。

摘　　要：【目的】观察野黄芩苷调节磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)/核因子kappa B(NF-κB)信号通路对溃疡性结肠炎小鼠结肠炎症损伤的影响。【方法】以葡聚硫酸钠(DSS)诱导建立溃疡性结肠炎(UC)小鼠模型。将造模成功的小鼠随机分为模型组、野黄芩苷组、YS-49(PI3K激活剂)组、野黄芩苷+YS-49组,每组12只;另取12只BALB/c小鼠设为正常组。各组给予相应干预后,对各组小鼠结肠炎症状进行疾病活动指数(DAI)评分,采用苏木精-伊红(HE)染色法观察各组小鼠结肠炎症损伤症状并进行评分,伊文思蓝(EB)法测定各组小鼠肠道通透性,酶联免疫吸附分析(ELISA)法检测血清结肠损伤标志物肠型脂肪酸结合蛋白(I-FABP)、二胺氧化酶(DAO)与促炎因子白细胞介素(IL)-1β、IL-17、肿瘤坏死因子α(TNF-α)水平,Western Blot法检测结肠组织炎性因子IL-1β、IL-17、TNF-α与PI3K/Akt/NF-κB通路蛋白表达。【结果】模型组小鼠DAI评分,结肠炎症评分,肠壁EB含量,血清I-FABP与DAO水平,血清IL-1β、IL-17与TNF-α含量,结肠组织IL-1β、IL-17与TNF-α蛋白表达水平,结肠组织p-PI3K/PI3K、p-Akt/Akt、p-NF-κB p65/NF-κB p65比值较正常组显著升高,差异均有统计学意义(P<0.05)。野黄芩苷组小鼠DAI评分,结肠炎症评分,肠壁EB含量,血清I-FABP与DAO水平,血清IL-1β、IL-17与TNF-α含量,结肠组织IL-1β、IL-17与TNF-α蛋白表达水平,结肠组织p-PI3K/PI3K、p-Akt/Akt、p-NF-κB p65/NF-κB p65比值较模型组降低,差异均有统计学意义(P<0.05);YS-49组小鼠DAI评分,肠壁EB含量,血清I-FABP与DAO水平,结肠炎症评分,血清IL-1β、IL-17与TNF-α含量,结肠组织IL-1β、IL-17与TNF-α蛋白表达水平,结肠组织p-PI3K/PI3K、p-Akt/Akt、p-NF-κB p65/NF-κB p65较模型组升高,差异均有统计学意义(P<0.05)。野黄芩苷+YS-49组小鼠DAI评分,肠壁EB含量,血清I-FABP与DAO水平,结肠炎症评分,血清IL-1β、IL-17与TNF-α含量,结肠组织IL-1β、IObjective To observe the effect of scutellarin on colonic inflammatory injury in mice with ulcerative colitis by regulating phosphatidylinositol-3 kinase(PI3K)/protein kinase B(Akt)/nuclear factor kappa B(NF-κB)signaling pathway.Methods Ulcerative colitis(UC)mouse model was induced by dextran sulfate sodium(DSS).The successfully modeled mice were randomly divided into model group,scutellarin group,YS-49(PI3 K activator)group and scutellarin+YS-49 group,with 12 mice in each group.Another 12 BALB/c mice were set as normal group.After the corresponding intervention was given to each group,the disease activity index(DAI)of colitis symptoms in each group was scored.Hematoxylin-eosin(HE)staining was used to observe and score the symptoms of colonic inflammatory injury in each group.Evans blue(EB)assay was used to determine the intestinal permeability of mice in each group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum colonic injury markers intestinal fatty acid binding protein(I-FABP),diamine oxidase(DAO)and pro-inflammatory factors interleukin(IL)-1β,IL-17 and tumor necrosis factorα(TNF-α).Western Blot was used to detect the expression of inflammatory factors IL-1β,IL-17,TNF-αand PI3K/Akt/NF-κB pathway proteins in colon tissue.Results The DAI score,colon inflammation score,EB content of intestinal wall,serum I-FABP and DAO levels,serum IL-1β,IL-17 and TNF-αlevels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3K/PI3K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 ratios in the model group were significantly higher than those in the normal group,and the differences were statistically significant(P<0.05).The DAI score,colon inflammation score,EB content in intestinal wall,serum I-FABP and DAO levels,serum IL-1β,IL-17 and TNF-αlevels,protein expression levels of colon tissue IL-1β,IL-17 and TNF-α,colon tissue p-PI3 K/PI3 K,p-Akt/Akt,p-NF-κB p65/NF-κB p65 ratios in the scutellarin group were lower than those in the model group,and the differences were

关 键 词：野黄芩苷 溃疡性结肠炎 PI3K/Akt/NF-κB通路 炎症 肠道屏障 小鼠 

分 类 号：R285.5[医药卫生—中药学]