野生大麦SOD基因家族生物信息学分析  

Bioinformatics Analysis of the Wild Barley SOD Gene Family

作　　者：高诚 林一欣 饶江雷 覃英 邓志军 GAO Cheng;LIN Yixin;RAO Jianglei;QIN Ying;DENG Zhijun(Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《湖北民族大学学报(自然科学版)》2025年第1期1-6,17,共7页Journal of Hubei Minzu University:Natural Science Edition

基　　金：国家自然科学基金项目(31860073);生物资源保护与利用湖北省重点实验室开放基金项目(KYPT012401)。

摘　　要：为了探究野生大麦(Hordeum vulgare ssp.spontaneum(K.Koch)Thell.)的抗逆潜力,通过生物信息学方法对其超氧化物歧化酶(superoxide dismutase,SOD)基因家族进行了全基因组水平的鉴定与分析。结果表明,野生大麦SOD基因家族共有10个成员,分属B1K_CSD、B1K_MSD、B1K_FSD 3个亚家族,分别有7、1、2个成员,不均匀分布在5条染色体上,与大麦相比增加了Horvu_FT11_3H01G447400、Horvu_FT11_5H01G364600、Horvu_FT11_5H01G368700 SOD基因。蛋白结构分析结果显示,野生大麦的SOD蛋白家族主要以无规则卷曲为主;同一蛋白亚家族成员的蛋白保守基序组成相似,在进化上相对保守,但基因结构差异较大。基因表达分析表明,在盐胁迫下,B1K_CSD1、B1K_CSD2、B1K_CSD6、B1K_CSD7、B1K_FSD1、B1K_FSD2、B1K_MSD17个基因的表达量发生了显著变化,在响应盐胁迫过程中起重要作用。野生大麦SOD基因家族的鉴定、分析对野生大麦SOD基因功能及盐胁迫响应机制研究具有一定的借鉴意义。To investigate the potential for resistance in wild barley(Hordeum vulgare ssp.spontaneum(K.Koch)Thell),the superoxide dismutase(SOD)gene family was identified and analyzed at the whole-genome level using bioinformatics methods.The results showed that the wild barley SOD gene family had ten members,belonging to three subfamilies of B1K_CSD,B1K_MSD and B1K_FSD,with seven,one and two members,respectively,unevenly distributed on five chromosomes,and compared with barley,Horvu_FT11_3H01G447400,Horvu_FT11_5H01G364600 and Horvu_FT11_5H01G368700 SOD genes were added.The structural analysis of proteins showed that wild barley SOD proteins were mainly composed of random coils.The protein conserved motifs of members of the same protein subfamily had similar composition and were relatively conserved in evolution,but there were significant differences in gene structure.Gene expression analysis indicated that the expression levels of seven genes,namely,B1K_CSD1,B1K_CSD2,B1K_CSD6,B1K_CSD7,B1K_FSD1,B1K_FSD2 and B1K_MSD1,changed significantly in response to salt stress and played an important role in the response to salt stress.The identification and analysis of the SOD gene family in wild barley could provide valuable insights into the function of SOD genes and the salt stress response mechanism in wild barley.

关 键 词：野生大麦 超氧化物歧化酶 基因家族 系统发育分析 基因表达 

分 类 号：S512.3[农业科学—作物学]