基于宏基因组学探究碳氮联合激发Arthrobacter参与关键碳代谢促进植物生长的研究  

Metagenomics reveals that carbon and nitrogen combination enhances Arthrobacter’s participation in critical carbon metabolism to promote plant growth

作　　者：刘超 秦仕华 闫茹 李仕蓉 许巧玲 乔志伟 LIU Chao;QIN Shi-hua;YAN Ru;LI Shi-rong;XU Qiao-ling;QIAO Zhi-wei(College of Resources and Environmental Engineering,Anshun University,Anshun Guizhou 561000;College of Agricultural,AnshunUniversity,Anshun Guizhou 561000;Rural Revitalization Research Center of Guizhou Universities,Anshun Guizhou561000)

机构地区：[1]安顺学院资源与环境工程学院,贵州安顺561000 [2]安顺学院农学院,贵州安顺561000 [3]贵州省高校乡村振兴研究中心,贵州安顺561000

出　　处：《中国土壤与肥料》2025年第1期53-61,共9页Soil and Fertilizer Sciences in China

基　　金：贵州省科技计划(黔科合基础ZK〔2023〕一般024);贵州省教育厅高等学校科学研究项目青年项目(黔教技〔2022〕327,黔教技〔2022〕331)。

摘　　要：土壤碳、氮养分对植物生长具有重要作用。为探究碳、氮对作物生长的影响机制,在黔中黄壤区通过田间盆栽试验,设置空白(CK)、葡萄糖(G)、尿素(U)、葡萄糖+尿素(GU)4个处理,分析外加碳、氮对作物生长、土壤微生物群落和代谢的影响。GU处理油菜鲜重、干重、茎粗等指标均显著高于其他处理(P<0.05),油菜株高也以GU处理为最高。G、U、GU处理土壤pH含量均低于CK处理,土壤全氮、碱解氮和有机质含量均高于CK处理。GU处理中土壤微生物的主要标志物种为节杆菌属(Arthrobacter),表明Arthrobacter在GU处理中可能发挥潜在的重要作用。KEGG Pathway levels 2各类代谢中,氮代谢和碳水化合物代谢占主导,其中,在氮循环代谢路径中,G处理固氮代谢途径占据主导,U处理硝化作用代谢途径占主导;在碳循环代谢路径中,GU处理碳水化合物代谢与其他3个处理有显著差异的途径有18条,CK、G、U分别有6、11、3条;GU处理18条显著差异代谢途径中,代谢基因主要来自Arothrobacter的有葡萄糖激酶glk(K00845)、核糖-5磷酸异构酶B rpiB(K01808)、1,6二磷酸果糖激酶glpX(K02446)、6磷酸-3己糖异构酶hxlB(K08094)、磷酸果糖激酶pfk和pfp(K21071)。结构方程模型表明,碳循环代谢基因对油菜生长和产量产生直接和显著的影响(P<0.001)。以上研究结果为从微生物角度探究碳、氮对作物生长的影响提供了研究基础,也为作物增产研究提供了理论支撑。Soil carbon and nitrogen nutrients play a crucial role in plant growth.To investigate the effects of carbon and nitrogen on crop growth,a field experiment was conducted in the yellow soil region of central Guizhou.Four treatments were established,including control(CK),glucose(G),urea(U),and glucose+urea(GU).The impacts of exogenous carbon and nitrogen on crop growth,soil microbial community,and metabolism were assessed.The GU treatment significantly increased the fresh weight,dry weight,and stem diameter of rapeseed compared to other treatments(P<0.05),and the plant height of rapeseed was also the highest in the GU treatment.The soil pH content of the G,U and GU treatments was lower than that of the CK treatment,while the soil total nitrogen,alkali-hydrolyzable nitrogen,and organic matter content were higher than those of the CK treatment.The predominant species of soil microbial community in the GU treatment was Arthrobacter,suggesting its potential importance in the GU treatment.At KEGG Pathway levels 2,nitrogen metabolism and carbohydrate metabolism were prominent.Among the nitrogen cycle metabolism pathways,the nitrogen fixation metabolism pathway dominated in the G treatment,while the nitrification metabolism pathway dominated in the U treatment.In the carbon cycle metabolism pathway,there were 18 significantly different metabolic pathways between the GU treatment and the other three treatments,with 6,11 and 3 pathways in CK,G and U,respectively.In the GU treatment,18 significantly different metabolic pathways were identified,with the metabolic genes mainly originating from Arthrobacter.These genes included glucose kinase glk(K00845),ribose-5-phosphate isomerase B rpiB(K01808),1,6-diphosphofructokinase glpX(K02446),6-phospho-3-hexulose isomerase hxlB(K08094),and phosphofructokinase pfk and pfp(K21071).Structural equation modeling demonstrated a direct and significant impact of carbon cycle metabolic genes on rapeseed growth and yield(P<0.001).This study laid the groundwork for investigating the influence of

关 键 词：外加碳氮 ARTHROBACTER 氮循环 碳循环 结构方程模型 

分 类 号：R28[医药卫生—中药学]