轻盈祛浊汤治疗糖尿病肾病的网络药理学机制分析  

Network pharmacological mechanism of Qingying Quzhuo decoction in the treatment of diabetic nephropathy

作　　者：白海龙 边云[1] 孟晓峰[2] 王丽芳[3] 王凤英 田风胜[1] 崔荣岗[1] 苏阳[1] 李娟[5] BAI Hailong;BIAN Yun;MENG Xiaofeng;WANG Lifang;WANG Fengying;TIAN Fengsheng;CUI Ronggang;SU Yang;LI Juan(Third Department of Diabetes,Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou,Hebei 061001,China;Preventive Care Center,Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou,Hebei 061001,China;Laboratory Department,Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou,Hebei 061001,China;Imaging Department,Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou,Hebei 061001,China;Nursing Department,Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou,Hebei 061001,China)

机构地区：[1]河北省沧州中西医结合医院糖尿病三科,河北沧州061001 [2]河北省沧州中西医结合医院治未病中心,河北沧州061001 [3]河北省沧州中西医结合医院检验科,河北沧州061001 [4]河北省沧州中西医结合医院影像科,河北沧州061001 [5]河北省沧州中西医结合医院护理部,河北沧州061001

出　　处：《安徽医药》2025年第4期671-676,I0001,共7页Anhui Medical and Pharmaceutical Journal

基　　金：河北省中医药管理局科研计划项目(2020465)。

摘　　要：目的探讨轻盈祛浊汤治疗糖尿病肾病(DN)的作用机制。方法利用网络药理学预测轻盈祛浊汤治疗DN的作用机制,并通过分子对接预测活性成分的结合部位。结合网络药理学及分子对接结果,构建DN大鼠模型,2023年1—6月,将60只大鼠分为正常组、模型组、马来酸依那普利组、轻盈祛浊汤组,每组15只。治疗8周后比较四组大鼠24 h尿微量白蛋白(urinary microalbuminexcretion rate,UAER)、尿素氮、血肌酐水平和钠/葡萄糖协同转运蛋白1(recombinant sodium/glucose cotransporter 1,SGLT1),A1腺苷受体(A1 adenosine receptor,A1AR)蛋白表达水平。结果网络药理学共筛选出轻盈祛浊汤有效成分1114种,作用靶点269个,DN相关靶点2020个,其交集靶点174个。基因本体富集(GO)和基因组的京都百科全书(KEGG)分析得出主要涉及信号转导、炎症反应、细胞凋亡等一系列的生物学反应过程,主要参与丝裂原活化蛋白激酶/核因子κB(mitogen-activated protein kinase/nuclear factor kappa-B,pMAPK/NF-κB)、NOD样受体家族蛋白3/白细胞介素-1β(NOD-like receptor protein 3/interleukin-1β,NLRP3/IL-1β)、白细胞介素6/信号传导和转录激活因子3(interleukin-6/signal transducer and activator of transcription 3,IL-6/STAT3)、肿瘤坏死因子(tumor necrosis factor,TNF)、肿瘤蛋白p53(tumor protein 53,P53)和前列腺素内过氧化物合酶(prostaglandin-endoperoxide synthase 2,PTGS2)等信号通路的调控。分子对接表明,轻盈祛浊汤主要成分与DN靶点的结合活性较强。模型组大鼠24 h UAER[(4539.71±516.03)μg/24 h比(226.59±72.71)μg/24 h]、血肌酐[(85.63±12.96)mL·kg^(-1)·min^(-1)比(0.48±0.12)mL·kg^(-1)·min^(-1)]、SGLT1(1.17±0.07比0.82±0.06)高于正常组,而模型组大鼠尿素氮、A1AR低于正常组(P<0.05)。马来酸依那普利片组大鼠24 h UAER、血肌酐、SGLT1低于模型组,马来酸依那普利片组大鼠尿素氮、A1AR高于模型组(P<0.05)。轻盈祛浊汤组大鼠24 h UAER�Objective To investigate the mechanism of Qingying Quzhuo decoction in the treatment of diabetic nephropathy.Methods Network pharmacology was used to predict the mechanism,and the binding sites of active ingredients were predicted by molecular docking.Combined with the results of network pharmacology and molecular docking,a rat model of diabetic nephropathy was established.From January to June 2023,60 rats were randomly assigned into normal group,model group,enalapril maleate group and Qingying Quzhuo decoction group,with 15 rats in each group.After 8 w of treatment,24 h urinary microalbuminexcretion rate(UAER),urea nitrogen,serum creatinine levels and protein expression levels of recombinant sodium/glucose cotransporter 1(SGLT1)and A1 adenosine receptor(A1AR).Results A total of 1114 kinds of active constituents of Qingying Quzhuo decoction were selected by network pharmacology,with 269 targets,2020 targets related to diabetic nephropathy and 174 intersection targets.GO and KEGG analysis results showed that it mainly involved a series of biological reaction processes such as signal transduction,inflammation and apoptosis,and involved primarily in the regulation of mitogen-activated protein kinase/nuclear factor kappa-B(pMAPK/NF-κB),NOD-like receptor protein 3/Interleukin-1β(NLRP3/IL-1β),interleukin-6/signal transducer and activator of transcription 3(IL-6/STAT3),tumor necrosis factor(TNF),tumor protein 53(P53)and prostaglandin-endoperoxide synthase 2(PTGS2)signaling pathways.Molecular docking showed that the main constituents of Qingying Quzhuo decoction had strong binding activity to the targets of diabetic nephropathy.Compared with normal group,24 h UAER[(4539.71±516.03)μg/24 h vs.(226.59±72.71)μg/24 h],serum creatinine[(85.63±12.96)mL·kg^(-1)·min^(-1)vs.(0.48±0.12)mL·kg^(-1)·min^(-1)]and SGLT1(1.17±0.07 vs.0.82±0.06)were increased in model group,while urea nitrogen and A1AR were decreased in model group(P<0.05).Compared with model group,24 h UAER,serum creatinine and SGLT1 were decreased in enal

关 键 词：糖尿病肾病 轻盈祛浊汤 痰湿瘀阻 网络药理学 作用机制 

分 类 号：R285.5[医药卫生—中药学]