Bifunctional nanoprobe for simultaneous detection of intracellular reactive oxygen species and temperature in single cells  

作　　者：Yanmei Ma Weikang Hu Jian Hu Muyang Ruan Jie Hu Ming Yang Yi Zhang Hanhan Xie Chengzhi Hu 

机构地区：[1]Shenzhen Key Laboratory of Biomimetic Robotics and Intelligent Systems,Department of Mechanical and Energy Engineering,Southern University of Science and Technology,Shenzhen 518000,China [2]Department of Electrical and Electronic Engineering,Southern University of Science and Technology,Shenzhen 518000,China

出　　处：《Microsystems & Nanoengineering》2024年第6期161-172,共12页微系统与纳米工程(英文)

基　　金：supported by the National Key Technologies R&D Program of China(Grant No.2023YFC2415900);Guangdong Basic and Applied Basic Research Foundation(No.2024A1515011915,No.2022A1515010098);partly supported by the Science,Technology,and Innovation Commission of Shenzhen Municipality under grant no.ZDSYS20200811143601004;in part by the Stable Support Plan Program of Shenzhen Natural Science Fund under grant 20220815104331001.

摘　　要：Living cells can rapidly adjust their metabolic activities in response to external stimuli,leading to fluctuations in intracellular temperature and reactive oxygen species(ROS)levels.Monitoring these parameters is essential for understanding cellular metabolism,particularly during dynamic biological processes.In this study,we present a bifunctional nanoprobe capable of simultaneous measurement of ROS levels and temperature within single cells.The nanoprobe features two individually addressable nanoelectrodes,with platinum(Pt)and nickel(Ni)coatings on both sides.At the tip,these two metal layers form a nano-thermocouple,enabling precise intracellular temperature measurements,while the Pt layer facilitates selective ROS detection.This dual functionality allows for real-time monitoring of cellular responses during synergistic chemo-photothermal therapy of cancer cells and zebrafish embryos subjected to mitochondrial toxic stress.Our results demonstrate that the nanoprobe effectively measures increases in temperature and ROS levels in HeLa cells undergoing chemo-photothermal therapy,as well as in chemically stimulated zebrafish embryos.By providing detailed analysis of submicrometer-scale temperature and ROS variations within living cells,this nanoprobe offers valuable insights into cellular processes and holds promise for early disease detection and drug development.

关 键 词：METABOLISM simultaneous holds 

分 类 号：Q503[生物学—生物化学] R318[医药卫生—生物医学工程]