A handheld HIV detection platform using paper-based sample preparation and real-time isothermal amplification  

作　　者：George Adedokun Gurjit Sidhu Morteza Alipanah Gary P.Wang Z.Hugh Fan 

机构地区：[1]Interdisciplinary Microsystems Group,Department of Mechanical&Aerospace Engineering,University of Florida,Gainesville,FL 32611,USA [2]Division of Infectious Diseases and Global Medicine,Department of Medicine,College of Medicine,University of Florida,Gainesville,FL 32610,USA [3]Medical Service,North Florida/South Georgia Veterans Health System,Gainesville,FL 32608,USA [4]Purrit Family Department of Biomedical Engineering,University of Florida,Gainesville,FL 32611,USA

出　　处：《Microsystems & Nanoengineering》2024年第6期213-223,共11页微系统与纳米工程(英文)

基　　金：supported in part by the University of Florida,USA(DRPDROF2020);the US National Institutes of Health(R01AI155735,R61AI181016,and R42AI122855);the Gatorade Trust through funds distributed by the University of Florida,Department of Medicine.

摘　　要：Early and accurate diagnosis of human immunodeficiency virus(HIV)infection is essential for timely initiation of antiretroviral therapy(ART)and prevention of new infections.However,conventional nucleic-acid-based tests for HIV detection require sophisticated laboratory equipment and trained personnel,which are often unavailable at the point-of-care(POC)or unaffordable in resource-limited settings.We report our development of a low-cost,integrated platform for POC testing of HIV.The platform integrates viral nucleic acid extraction on a paper substrate and reverse transcription loop-mediated isothermal amplification(RT-LAMP)in a portable,battery-powered heating device with real-time detection.The platform does not require laboratory infrastructure such as power outlets.The assay showed a detection limit of 30 copies/mL of HIV RNA in 140μL human serum or 4 copies/reaction using 50μL human serum,with no cross-reactivity with hepatitis C virus(HCV).We validated the platform using both plasma samples spiked with HIV and clinical samples from HIV-positive individuals,and compared it with standard laboratory assays based on polymerase chain reaction(PCR).These results demonstrate the feasibility of our platform for HIV testing at the POC.

关 键 词：PREVENTION testing ISOTHERMAL 

分 类 号：R51[医药卫生—内科学]